Dar K K, Ali S, Ejaz M, Nasreen S, Ashraf N, Gillani S F, Shafi N, Safeer S, Khan M A, Andleeb S, Mughal T A
College of Life Science and Technology, Beijing University of Chemical Technology, 100029, Beijing, China.
Department of Zoology, Government College University Lahore, Lahore-54000, Pakistan.
Braz J Biol. 2019 Oct-Dec;79(4):629-638. doi: 10.1590/1519-6984.186565. Epub 2019 Jan 14.
Hepatocellular carcinoma is the most frequent primary malignancy of liver and accounts for as many as one million deaths worldwide in a year.
The aim of the present study was to evaluate the anti-cancerous efficiency of Bergenia ciliata rhizome against diethylnitrosoamine induced hepatocarcinogenesis in Balb C mice.
One percent diethylnitrosoamine was prepared by using 99 ml of normal saline NaCl (0.9 percent) solution to which was added 1 ml of concentrated diethylnitrosoamine (DEN) solution (0.01 μg/μl). Extract of Bergenia ciliata was prepared by maceration technique. Mice were classified into four groups as follows: Group 1 a control group (N=7) received saline solution (3.5 μl/mg), group 2 (N=14) received diethylnitrosoamine (3.5 μl/mg) intraperitoneally once in a week for eight consecutive weeks, group 3 (N=7) received plant extract (150 mg/kg (Body weight)) once in a week, while group 4 (N=7) was given combination of diethylnitrosoamine (3.5 μl/mg) and plant extract (150 mg/kg (Body weight)). After eight weeks of DEN induction group 2 mice were divided into two subgroups containing seven mice each, subgroup 1 was sacrificed while subgroup 2 was treated with plant extract (150 mg/kg (Body weight)) once in a week for eight consecutive weeks.
The model of DEN injected hepatocellular carcinomic (HCC) mice elicited significant decline in levels of albumin with concomitant significant elevations in tumor markers aspartate aminotransferase, alanine aminotransferase (ALT), lactate dehydrogenase (LDH), alpha feto protein (AFP), gamma glutamyl transferase (Y-GT), 5 nucleotidase (5NT), glucose-6-phosphate dehydrogenase (G6PDH) and bilirubin. The intraperitoneal administration of B. ciliata as a protective agent, produced significant increase in albumin levels with significant decrease in the levels of tumor markers aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), alpha feto protein (AFP), gamma glutamyl transferase (Y-GT), 5 nucleotidase (5NT), glucose-6-phosphate dehydrogenase (G6PDH) and bilirubin.
Bergenia ciliata has potent antioxidant activity, radical scavenging capacity and anticancerous properties. Bergenia ciliata extracts may provide a basis for development of anti-cancerous drug.
肝细胞癌是肝脏最常见的原发性恶性肿瘤，全球每年有多达100万人死于该病。
本研究旨在评估岩白菜根茎对二乙基亚硝胺诱导的Balb C小鼠肝癌发生的抗癌效果。
用99 ml生理盐水（0.9%）溶液加入1 ml浓缩二乙基亚硝胺（DEN）溶液（0.01 μg/μl）配制1%的二乙基亚硝胺。采用浸渍技术制备岩白菜提取物。将小鼠分为四组如下：第1组为对照组（N = 7），接受生理盐水溶液（3.5 μl/mg）；第2组（N = 14）连续8周每周一次腹腔注射二乙基亚硝胺（3.5 μl/mg）；第3组（N = 7）每周一次接受植物提取物（150 mg/kg（体重））；第4组（N = 7）给予二乙基亚硝胺（3.5 μl/mg）和植物提取物（150 mg/kg（体重））的组合。在二乙基亚硝胺诱导8周后，将第2组小鼠分为两个亚组，每组7只，处死亚组1，亚组2连续8周每周一次接受植物提取物（150 mg/kg（体重））治疗。
注射二乙基亚硝胺的肝细胞癌（HCC）小鼠模型导致白蛋白水平显著下降，同时肿瘤标志物天冬氨酸转氨酶、丙氨酸转氨酶（ALT）、乳酸脱氢酶（LDH）、甲胎蛋白（AFP）、γ-谷氨酰转移酶（Y-GT）、5-核苷酸酶（5NT）、葡萄糖-6-磷酸脱氢酶（G6PDH）和胆红素显著升高。腹腔注射岩白菜作为保护剂，可使白蛋白水平显著升高，肿瘤标志物天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、乳酸脱氢酶（LDH）、甲胎蛋白（AFP）、γ-谷氨酰转移酶（Y-GT）、5-核苷酸酶（5NT）、葡萄糖-6-磷酸脱氢酶（G6PDH）和胆红素水平显著降低。
岩白菜具有强大的抗氧化活性、自由基清除能力和抗癌特性。岩白菜提取物可能为抗癌药物的开发提供基础。
