Alves C C F, Oliveira J D, Estevam E B B, Xavier M N, Nicolella H D, Furtado R A, Tavares D C, Miranda M L D
Instituto Federal de Educação, Ciência e Tecnologia Goiano, Campus Rio Verde, Av. Sul Goiana, s/n, Zona Rural, CEP 75901-970, Rio Verde, GO, Brasil.
Centro de Pesquisa em Ciências Exatas e Tecnologia, Universidade de Franca, Av. Dr. Armando de Salles Oliveira, 201, Parque Universitário, CEP 14404-600, Franca, SP, Brasil.
Braz J Biol. 2020 Apr-Jun;80(2):290-294. doi: 10.1590/1519-6984.192643.
Essential oils, which may be extracted from several parts of plants, have different biological activities. The Brazilian Cerrado has a large variety of plants that yield essential oils, even though many have not been studied yet. Taking into account the biodiversity of this biome, this study aimed at evaluating the antiproliferative activity of essential oils extracted from three species of plants of the Cerrado in Goiás state: Campomanesia adamantium (Cambess.) O. Berg, Protium ovatum (Engl. in Mart.) and Cardiopetalum calophyllum (Schltdl.). Essential oils were extracted from both C. adamantium and C. calophyllum leaves and from P. ovatum leaves and green fruits by hydrodistillation carried out by a Clevenger-type apparatus. The chemical composition of the essential oils was determined by Gas Chromatography coupled to Mass Spectrometry (GC-MS). The following major chemical constituents were identified in the essential oils under investigation: β-myrcene (62.00%), spathulenol (28.78%), germacrene-B (18.27%), β-caryophyllene oxide (16.40%), β-caryophyllene (14.00%), α-pinene (11.30%), viridiflorol (9.99%), limonene (7.30%) and (Z,E)-pharnesol (6.51%). The antiproliferative activity was evaluated in different human tumor cell lines: breast adenocarcinoma (MCF-7), cervical adenocarcinoma (HeLa) and glioblastoma (M059J). A normal human cell line was included (GM07492A, lung fibroblasts). Results showed that essential oils from C. adamantium leaves got the lowest values of IC50 in all strains of tumor cells under evaluation. They were significantly lower than the ones of the normal cell line, an evidence of selectivity. It is worth mentioning that this is the first report of the antiproliferative activity of essential oils from C. adamantium , P. ovatum and C. calophyllum against human tumor cells.
精油可从植物的多个部位提取，具有不同的生物活性。巴西塞拉多地区有各种各样能产生精油的植物，尽管许多植物尚未得到研究。考虑到该生物群落的生物多样性，本研究旨在评估从戈亚斯州塞拉多地区的三种植物中提取的精油的抗增殖活性，这三种植物分别是：金钟番樱桃（Campomanesia adamantium (Cambess.) O. Berg）、卵叶原檀（Protium ovatum (Engl. in Mart.)）和心叶瓣蕊花（Cardiopetalum calophyllum (Schltdl.)）。通过使用克利夫enger型仪器进行水蒸馏，从金钟番樱桃和心叶瓣蕊花的叶子以及卵叶原檀的叶子和绿色果实中提取精油。通过气相色谱 - 质谱联用仪（GC - MS）测定精油的化学成分。在所研究的精油中鉴定出以下主要化学成分：β - 月桂烯（62.00%）、匙叶桉油烯醇（28.78%）、吉马烯 - B（18.27%）、β - 石竹烯氧化物（16.40%）、β - 石竹烯（14.00%）、α - 蒎烯（11.30%）、绿花白千层醇（9.99%）、柠檬烯（7.30%）和（Z,E）- 法尼醇（6.51%）。在不同的人类肿瘤细胞系中评估抗增殖活性：乳腺腺癌（MCF - 7）、宫颈腺癌（HeLa）和成胶质细胞瘤（M059J）。纳入了一种正常人类细胞系（GM07492A，肺成纤维细胞）。结果表明，在所有评估的肿瘤细胞系中，金钟番樱桃叶精油的半数抑制浓度（IC50）值最低。它们显著低于正常细胞系的值，这是选择性的一个证据。值得一提的是，这是关于金钟番樱桃、卵叶原檀和心叶瓣蕊花精油对人类肿瘤细胞抗增殖活性的首次报道。
