Department of Anatomy, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India.
Department of Orthopedics, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India.
Scand J Immunol. 2019 Jul;90(1):e12770. doi: 10.1111/sji.12770. Epub 2019 May 14.
Increasing evidence suggests a role of inflammation during the pathogenesis of osteoarthritis (OA). The local and systemic inflammation was studied in 33 patients of different KL grades, grade2 (n = 11), grade3 (n = 6) and grade4 (n = 16). The levels of cytokines, adipokines and matrix metalloproteinases (MMPs) were measured in serum and synovial fluid (SF) by flow cytometry and ELISA, respectively. The frequency of T cells and CD161 expression was measured by flow cytometry. The levels of IL-1β, IL-6 and IL-10 were significantly higher in sera and SF of patients with OA as compared to healthy control's serum. Higher levels of MMP9 and leptin and lower levels of adiponectin were observed in SF as compared to serum. The MMP9 in SF and MMP13 levels in serum and SF decreased in KL grade 4 cases. In these patients, higher levels of leptin and lower levels of adiponectin were observed in SF versus patients of lower grades. There was increased infiltration of CD8+ T cells in SF of OA cases with decreased frequency in grade 4 cases. The expression of CD161 on T cells was significantly higher in SF than peripheral blood with significant upregulation in grade 4 patients. The CD161 expression had significant positive correlation with IL-17 in the serum of patients. The ROC curves of CD161 expression significantly distinguished grade 2 and grade 4 patients. Collectively, an elevated CD161 expression on T cells in circulation and synovial compartment clearly distinguished lower and higher grade patients warranting studies to assess its role as a contributing factor towards OA progression.
越来越多的证据表明炎症在骨关节炎（OA）发病机制中起作用。研究了 33 名不同 KL 分级的患者的局部和全身炎症，2 级（n=11），3 级（n=6）和 4 级（n=16）。通过流式细胞术和 ELISA 分别在血清和滑液（SF）中测量细胞因子、脂肪因子和基质金属蛋白酶（MMPs）的水平。通过流式细胞术测量 T 细胞和 CD161 表达的频率。与健康对照组血清相比，OA 患者血清和 SF 中的 IL-1β、IL-6 和 IL-10 水平显著升高。SF 中 MMP9 和瘦素水平较高，而血清中脂联素水平较低。SF 中 MMP9 和血清及 SF 中 MMP13 水平在 KL 4 级病例中降低。在这些患者中，SF 中的瘦素水平较高，而 SF 和血清中的脂联素水平较低。OA 患者 SF 中 CD8+T 细胞浸润增加，4 级患者中 CD8+T 细胞频率降低。与外周血相比，SF 中 T 细胞上的 CD161 表达显著升高，4 级患者的表达显著上调。患者血清中 CD161 表达与 IL-17 呈显著正相关。CD161 表达的 ROC 曲线可显著区分 2 级和 4 级患者。总之，循环和滑膜隔间中 T 细胞上 CD161 表达的升高可明显区分低级别和高级别患者，需要进一步研究以评估其作为 OA 进展的促成因素的作用。
