Jarecki Jaromir, Małecka-Massalska Teresa, Polkowska Izabela, Potoczniak Bartosz, Kosior-Jarecka Ewa, Szerb Imre, Tomaszewska Ewa, Gutbier Martina, Dobrzyński Maciej, Blicharski Tomasz
Department of Rehabilitation and Orthopaedics, Medical University of Lublin, 20-059 Lublin, Poland.
Department of Orthopaedics and Traumatology, Regional Hospital in Chełm, 22-100 Chełm, Poland.
J Clin Med. 2021 Mar 18;10(6):1263. doi: 10.3390/jcm10061263.
The aim of this study was to assess levels of adiponectin (ACRP-30) and leptin in serum and synovial fluid (SF) of overweight females with primary gonarthrosis (GOA) and to look for their correlations with clinical status and the level of biochemical OA biomarkers: matrix metalloproteinase (MMP) 1, MMP-9 and tissue inhibitor of metalloproteinase (TIMP-1). The studied group consisted of 39 overweight females undergoing primary total knee arthroplasty as a result of GOA. The stage of GOA was evaluated according to Knee Society Score (KSS), Ahlbäck and Kellgren-Lawrence (K-L) scores. Peripheral blood and SF were obtained. Levels of selected biomarkers were assessed using ELISA kits. The mean level of ACRP-30 in serum valued were 8393.80 ng/mL and in SF, 774.33 ng/mL, the mean concentration of leptin in serum was 32,040.74 pg/mL and in SF, 27,332.12 pg/mL. Levels of leptin in serum and SF correlated with body mass index (BMI), ( = 0.0005, and = 0.0002, respectively). Levels of ACRP-30 in serum was correlated with clinical scores (Ahlbäck: = 0.0214; K-L: = 0.0146). ACRP-30 in SF correlated with ACRP-30 in serum ( = 0.0003), tended to negatively correlate with MMP-1 in serum ( = 0.0598) and positively correlate with pro-MMP-1 in SF ( = 0.0600). To conclude, this study confirms the correlations between concentrations of both, leptin and ACRP-30, comparing serum and SF. In overweight females, leptin levels increase with BMI and ACRP-30 serum level increase in more advanced GOA stages. Finally, leptin levels were correlated with TIMP-1 serum concentration, one of the biochemical markers of GOA.
本研究旨在评估原发性膝关节骨性关节炎（GOA）超重女性血清和滑液（SF）中脂联素（ACRP - 30）和瘦素水平，并探寻它们与临床状况以及生化性骨关节炎生物标志物水平的相关性：基质金属蛋白酶（MMP）1、MMP - 9和金属蛋白酶组织抑制剂（TIMP - 1）。研究组由39例因GOA接受初次全膝关节置换术的超重女性组成。根据膝关节协会评分（KSS）、阿尔贝克评分和凯尔格伦 - 劳伦斯（K - L）评分对GOA分期进行评估。采集外周血和滑液。使用酶联免疫吸附测定试剂盒评估所选生物标志物的水平。血清中ACRP - 30的平均水平为8393.80 ng/mL，滑液中为774.33 ng/mL；血清中瘦素的平均浓度为32,040.74 pg/mL，滑液中为27,332.12 pg/mL。血清和滑液中瘦素水平与体重指数（BMI）相关（分别为 = 0.0005和 = 0.0002）。血清中ACRP - 30水平与临床评分相关（阿尔贝克评分： = 0.0214；K - L评分： = 0.0146）。滑液中的ACRP - 30与血清中的ACRP - 30相关（ = 0.0003），与血清中的MMP - 1呈负相关趋势（ = 0.0598），与滑液中的前MMP - 1呈正相关（ = 0.0600）。总之，本研究证实了血清和滑液中瘦素与ACRP - 30浓度之间的相关性。在超重女性中，瘦素水平随BMI升高而增加，ACRP - 30血清水平在更晚期的GOA阶段升高。最后，瘦素水平与GOA的生化标志物之一TIMP - 1血清浓度相关。
