Department of Clinical Immunology, PLA Specialized Research Institute of Rheumatology & Immunology, Xijing Hospital, Fourth Military Medical University, 15 West Changle Road, Xi'an, 710032, China.
Department of Hemotalogy and Rheumatology, Hanzhong 3201 Hospital, Hanzhong, 723000, China.
Clin Rheumatol. 2018 Jan;37(1):59-66. doi: 10.1007/s10067-017-3800-9. Epub 2017 Sep 25.
CD161 is used as a surrogate marker for Th17 cells, which are implicated in the pathogenesis of rheumatoid arthritis (RA). In this study, we evaluated the percentage, clinical significance, and CD98 and CD147 expression of CD4CD161 T cells. The potential role of CD147 and CD98 in cyclophilin A-induced chemotaxis of CD4CD161 T cells was analyzed. Thirty-seven RA patients, 15 paired synovial fluid (SF) of RA, and 22 healthy controls were recruited. The cell populations and surface expression of CD98 and CD147 were analyzed by flow cytometry. Spearman's rank correlation coefficient and multiple linear regression were applied to calculate the correlations. Chemotaxis assay was used to investigate CD4CD161 T cell migration. We found that the percentage of CD4CD161 T cells and their expression of CD147 and CD98 in SF were higher than in the peripheral blood of RA patients. Percentage of SF CD4CD161 T cells was positively correlated with 28-Joint Disease Activity Score (DAS28). CD147 monoclonal antibody (HAb18) attenuated the chemotactic ability of CD4CD161 T cells. An increased CD4CD161 T cell percentage and expression of CD147 and CD98 were shown in RA SF. Percentage of SF CD4CD161 T cells can be used as a predictive marker of disease activity in RA. CD147 block significantly decreased the chemotactic index of CD4CD161 cells induced by cyclophilin A (CypA). These results imply that the accumulation of CD4CD161 T cells in SF and their high expression of CD147 may be associated with CypA-mediated chemotaxis and contribute to local inflammation in RA.
CD161 被用作 Th17 细胞的替代标志物，Th17 细胞参与类风湿关节炎（RA）的发病机制。在这项研究中，我们评估了 CD4+CD161+T 细胞的百分比、临床意义以及 CD98 和 CD147 的表达。分析了 CD147 和 CD98 在亲环素 A 诱导 CD4+CD161+T 细胞趋化中的潜在作用。招募了 37 名 RA 患者、15 对 RA 关节液（SF）和 22 名健康对照者。通过流式细胞术分析细胞群和 CD98 和 CD147 的表面表达。应用 Spearman 秩相关系数和多元线性回归计算相关性。趋化实验用于研究 CD4+CD161+T 细胞迁移。我们发现，RA 患者 SF 中 CD4+CD161+T 细胞的百分比及其 CD147 和 CD98 的表达高于外周血。SF CD4+CD161+T 细胞的百分比与 28 关节疾病活动评分（DAS28）呈正相关。CD147 单克隆抗体（HAb18）减弱了 CD4+CD161+T 细胞的趋化能力。RA SF 中显示出 CD4+CD161+T 细胞百分比增加和 CD147 和 CD98 的表达增加。SF CD4+CD161+T 细胞的百分比可作为 RA 疾病活动的预测标志物。CD147 阻断显著降低了亲环素 A（CypA）诱导的 CD4+CD161 细胞的趋化指数。这些结果表明，SF 中 CD4+CD161+T 细胞的积累及其 CD147 的高表达可能与 CypA 介导的趋化作用有关，并有助于 RA 中的局部炎症。
