School of Life Sciences, SRTM University, Nanded, Maharashtra, 431606, India.
Department of Stem Cell and Regenerative medicine, Centre For Interdisciplinary Research, DY Patil Education Society (Deemed to be University), Kolhapur, Maharashtra, 416006, India.
J Antibiot (Tokyo). 2019 Aug;72(8):640-644. doi: 10.1038/s41429-019-0185-9. Epub 2019 Apr 24.
We have analyzed the expressions of genes which regulate Ras-cAMP-EFG1 and CEK1-MAPK pathways involved in yeast to hyphal form morphogenesis in Candida albicans. The expression profile of genes associated with serum-induced morphogenesis showed reduced expressions of genes involved in these pathways by the treatment with biofabricated silver nanoparticles. Cell elongation gene, ECE1, was downregulated by 5.1 fold by the treatment of silver nanoparticles. Expression of hyphal inducer gene, TEC1 was downregulated by 6.28 fold. Negative regulators of yeast to hyphal transition, TUP1 and RFG1 were downregulated by 2.45 and 5.43 fold, respectively. Current study suggests that silver nanoparticles affect gene expression and may subsequently reduce virulence in C. albicans. Targeting genes involved in virulence may be an acceptable novel treatment strategy for pathogenic fungal infections.
我们分析了参与白念珠菌从酵母相到菌丝相形态发生的 Ras-cAMP-EFG1 和 CEK1-MAPK 途径相关基因的表达。与血清诱导形态发生相关的基因表达谱显示，生物制造的银纳米粒子处理后，这些途径相关基因的表达减少。细胞伸长基因 ECE1 的表达被银纳米粒子处理下调了 5.1 倍。菌丝诱导基因 TEC1 的表达被下调了 6.28 倍。酵母到菌丝转变的负调控因子 TUP1 和 RFG1 的表达分别下调了 2.45 倍和 5.43 倍。目前的研究表明，银纳米粒子影响基因表达，从而可能降低白念珠菌的毒力。针对与毒力相关的基因可能是一种可接受的治疗致病性真菌感染的新策略。
