Ramotar D, Newman E B
Can J Microbiol. 1986 Nov;32(11):842-6. doi: 10.1139/m86-155.
We have shown that serine-glycine auxotrophs of Escherichia coli K-12 use exogenous L-serine inefficiently as a source of biosynthetic intermediates. Much of the L-serine supplied in the medium is not used to satisfy the auxotrophic requirement, owing to its diversion by L-serine deaminase, presumably to pyruvate. This is the first proof that the activity known as L-serine deaminase actually deaminates L-serine in vivo.
我们已经表明，大肠杆菌K-12的丝氨酸-甘氨酸营养缺陷型菌株将外源L-丝氨酸作为生物合成中间体的来源时利用效率低下。培养基中提供的大部分L-丝氨酸并未用于满足营养缺陷型的需求，因为它被L-丝氨酸脱氨酶转化，推测转化为丙酮酸。这是首个证据，证明被称为L-丝氨酸脱氨酶的活性在体内确实能使L-丝氨酸脱氨。
