Roghmann M C, Skinner K A, Hilf R
Cancer Res. 1987 Mar 1;47(5):1348-53.
The effects of altered estrogenic environments on creatine kinase (CK) and adenylate kinase (AK) were studied in two rodent mammary tumor systems, R3230AC and primary 7,12-dimethylbenz(a)anthracene (DMBA)-induced carcinomas, to determine whether response of these enzymes could be related to their hormone dependence. The hormonal perturbations studied were ovariectomy and administration of various doses of estradiol valerate or the antiestrogen tamoxifen. Total CK activity and AK activity were assessed by a spectrophotometric assay followed by electrophoretic separation of the CK isozymes to determine their relative activities. In the ovarian-independent R3230AC tumors, estrogen treatment produced a dose-related decrease in CK activity, whereas CK was not responsive in ovarian-dependent DMBA-induced tumors. Adenylate kinase activity remained unchanged regardless of the hormonal perturbation. Glucose-6-phosphate dehydrogenase and lactate dehydrogenase, which were studied for comparative purposes, were both estrogen responsive. While both estrogenic and antiestrogenic effects on enzyme activities were observed in the DMBA-induced tumors, the effect of tamoxifen in the R3230AC tumors was generally estrogenic. We conclude that the effect of estrogen on CK-BB in DMBA-induced tumors is not sufficient to be used as a biochemical marker of hormone dependence.
在两种啮齿动物乳腺肿瘤模型，即R3230AC和原发性7,12 - 二甲基苯并(a)蒽（DMBA）诱导的癌中，研究了雌激素环境改变对肌酸激酶（CK）和腺苷酸激酶（AK）的影响，以确定这些酶的反应是否与其激素依赖性有关。所研究的激素干扰包括卵巢切除术以及给予不同剂量的戊酸雌二醇或抗雌激素他莫昔芬。通过分光光度法测定总CK活性和AK活性，随后对CK同工酶进行电泳分离以确定其相对活性。在不依赖卵巢的R3230AC肿瘤中，雌激素治疗使CK活性呈剂量相关下降，而在依赖卵巢的DMBA诱导的肿瘤中CK无反应。无论激素干扰如何，腺苷酸激酶活性均保持不变。为作比较而研究的葡萄糖-6-磷酸脱氢酶和乳酸脱氢酶均对雌激素有反应。虽然在DMBA诱导的肿瘤中观察到了雌激素和抗雌激素对酶活性的影响，但他莫昔芬对R3230AC肿瘤的影响通常是雌激素样的。我们得出结论，雌激素对DMBA诱导肿瘤中CK - BB的影响不足以用作激素依赖性的生化标志物。
