Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, 430030, PR China.
Wuhan Tongji Reproductive Medicine Hospital, Wuhan, 430030, PR China.
Epigenomics. 2019 Jun;11(8):935-949. doi: 10.2217/epi-2018-0217. Epub 2019 Apr 25.
To identify the circRNAs expression pattern and roles in bisphenol A (BPA) induced germ cell apoptosis. We performed circRNA/miRNA/mRNA-Seq in 120 μM BPA treated and nontreated GC-2 cells. Bioinformatic analysis, qPCR, apoptosis assays, luciferase report were done in the function analysis. A large number of apoptosis related circRNAs/miRNAs/mRNAs were differentially expressed with competing endogenous RNA network constructed. Interestingly, most investigated upregulated circRNAs, including circDcbld2, circMapk1, circMpp6 and circTbc1d20 showed protective effects in antagonizing BPA toxicity, with the effects individually and synergistically observed. CircMapk1 may take its role by sponging miR-214-3p. circRNAs can play protective roles via sponging miRNAs in toxicity. Some circRNAs may serve as novel targets for BPA toxicity intervention or as biomarkers.
为了鉴定双酚 A（BPA）诱导生殖细胞凋亡过程中的 circRNA 表达模式和作用。我们对 120 μM BPA 处理和未处理的 GC-2 细胞进行了 circRNA/miRNA/mRNA-Seq 分析。通过生物信息学分析、qPCR、细胞凋亡检测和荧光素酶报告实验进行了功能分析。构建了竞争性内源 RNA 网络，发现大量与细胞凋亡相关的 circRNAs/miRNAs/mRNAs 存在差异表达。有趣的是，大多数上调的 circRNAs，包括 circDcbld2、circMapk1、circMpp6 和 circTbc1d20，在拮抗 BPA 毒性方面表现出保护作用，单独或协同观察到这种作用。CircMapk1 可能通过海绵吸附 miR-214-3p 发挥作用。circRNAs 可以通过海绵吸附 miRNA 发挥毒性保护作用。一些 circRNAs 可能作为 BPA 毒性干预的新型靶点或作为生物标志物。
