Department of Diagnosis and Surgery, São Paulo State University (UNESP), School of Dentistry, Araraquara, Brazil.
Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), School of Dentistry, Araraquara, Brazil.
Clin Oral Implants Res. 2019 Jul;30(7):603-616. doi: 10.1111/clr.13445. Epub 2019 May 11.
To evaluate the effect of Cisplatin on bone repair and mineralization around implants and on the mechanical properties of bone tissue.
Forty-three Wistar rats were randomly divided into two groups: Cisplatin (CIS, medication) and control (CTL, placebo solution), administered once a week for 4 weeks. After 4 weeks, implants were installed in both tibiae metaphysis. After 30 and 60 days, the animals were sacrificed and their femurs and tibiae were removed. Femurs were subjected to mechanical tests and tibiae for removal torque, arrangement and distribution of collagen fibers, morphometrical analyses (bone tissue in contact with the implant surface [BIC] and areal fraction between implant threads occupied by bone tissue [BAFO]) and scanning electron microscopy to calcium and calcium/phosphorus analysis. Data were analyzed by ANOVA or MANOVA, and Tukey or Games-Howell post hoc tests, respectively (α = 0.05).
The CTL specimens had significantly higher values (0.0001 ≤ p≤0.036) of strength (N), removal torque (N/cm ), %BIC, and %BAFO than CIS specimens, being their best results at day 60. No significant differences were found among the groups regarding the values of deformation, percentage of calcium, and calcium/phosphorus ratio. In CIS groups, there was a reduction in the organization of collagen at the bone/implant interface, resulting in a trabecular bone with thin trabeculae and birefringent collagen and irregular arrangement.
Cisplatin interfered negatively in the repair and mineralization around dental implants, as well as on the quality of the bone tissue, mainly in the period of 30 days after the implant placement.
评估顺铂对种植体周围骨修复和矿化以及骨组织力学性能的影响。
43 只 Wistar 大鼠随机分为两组：顺铂（CIS，药物）组和对照组（CTL，安慰剂溶液），每周给药 1 次，共 4 周。4 周后，在双侧胫骨干骺端植入种植体。30 天和 60 天后处死动物，取出其股骨和胫骨。对股骨进行力学测试，对胫骨进行扭矩移除、胶原纤维排列和分布、形态计量学分析（与植入物表面接触的骨组织 [BIC]和植入物螺纹之间被骨组织占据的面积分数 [BAFO]）和扫描电镜分析，以进行钙和钙/磷分析。采用方差分析或多变量方差分析，以及 Tukey 或 Games-Howell 事后检验分别进行数据分析（α=0.05）。
CTL 标本的强度（N）、扭矩移除（N/cm）、BIC%和 BAFO%值明显高于 CIS 标本（0.0001≤p≤0.036），最佳结果出现在 60 天。各组间的变形值、钙含量百分比和钙/磷比值无显著差异。在 CIS 组中，骨/植入物界面的胶原组织减少，导致小梁骨的小梁变薄、双折射胶原和排列不规则。
顺铂对种植体周围的修复和矿化以及骨组织质量产生负面影响，主要发生在植入物放置后 30 天内。
