Tedder T F, Schmidt R E, Rudd C E, Kornacki M M, Ritz J, Schlossman S F
Eur J Immunol. 1986 Dec;16(12):1539-43. doi: 10.1002/eji.1830161212.
Activated T lymphocytes can provide all of the signals necessary to induce the proliferation of resting B lymphocytes. The activation signal is presumably initiated through direct T-B lymphocyte contact. The role of the leukocyte function antigen-1 (LFA-1) and T4 molecules in the activation of purified, small B lymphocytes by mitomycin C-treated T lymphocytes was examined by using monoclonal antibodies that react with and inhibit the function of these molecules. Anti-LFA-1 antibody binding significantly inhibited T-B lymphocyte interactions that result in B lymphocyte proliferation. In contrast, the presence of anti-T4 antibodies at concentrations as high as 100 micrograms/ml did not inhibit this interaction. These results indicate that the B lymphocyte activation signal may not be mediated through the interaction of T4 molecules with major histocompatibility complex class II antigens of the B lymphocyte but is a cell-cell contact-dependent event that is facilitated by LFA-1 molecules.
活化的T淋巴细胞能提供诱导静止B淋巴细胞增殖所需的所有信号。活化信号大概是通过T细胞与B淋巴细胞的直接接触启动的。利用与白细胞功能抗原-1（LFA-1）和T4分子反应并抑制其功能的单克隆抗体，研究了LFA-1和T4分子在经丝裂霉素C处理的T淋巴细胞激活纯化的小B淋巴细胞中的作用。抗LFA-1抗体结合显著抑制了导致B淋巴细胞增殖的T-B淋巴细胞相互作用。相比之下，浓度高达100微克/毫升的抗T4抗体的存在并未抑制这种相互作用。这些结果表明，B淋巴细胞活化信号可能不是通过T4分子与B淋巴细胞主要组织相容性复合体II类抗原的相互作用介导的，而是一个依赖细胞间接触的事件，LFA-1分子促进了这一事件。
