Futagami-Mizoguchi E, Yamada A, Mizoguchi A, Imai Y, Yokoyama M M
Department of Immunology, Kurume University School of Medicine, Japan.
Am J Pathol. 1993 Sep;143(3):672-7.
The lymphocyte function associated antigen 1 has been found only on leukocytes and lymphoid tissues; however, the expression of lymphocyte function associated antigen 1 on nonhematopoietic cells has not been reported previously. In this study, immunohistochemical expression of lymphocyte function associated antigen 1 was examined on various tissues from 35 patients with malignant diseases and 36 patients with benign diseases including benign tumors. The expression of lymphocyte function associated antigen 1 was found on various exocrine tissues (eg, gastric glands, bronchial epithelium, alveolar epithelium, duodenal glands, bile ducts, pancreatic acini, and salivary glands) uninvolved by tumor in patients with malignant diseases. Localization of lymphocyte function associated antigen 1 was limited to the exocrine glands and differed from tissue-infiltrating leukocytes. The expression of lymphocyte function associated antigen 1 on exocrine tissues was confirmed in all 35 cases of malignant diseases that were examined. These included a wide spectrum of carcinomas and hematopoietic tumors. In contrast, none of the 36 cases with benign diseases examined expressed lymphocyte function associated antigen 1 on their exocrine glands. These results indicate a strong correlation between lymphocyte function associated antigen 1 expression on exocrine glands and malignant disease. The expression of lymphocyte function associated antigen 1 on nonhematopoietic cells was further confirmed in nonhematopoietic cell lines. Two of 19 nonhematopoietic cell lines (MKN45 and PANC-1; exocrine gland cell lines) examined expressed lymphocyte function associated antigen 1 on both cell surface and cytoplasm. These results suggested that immunohistochemically defined lymphocyte function associated antigen 1 molecules on nontumorous exocrine gland cells are a potential marker for the presence of malignant diseases.
淋巴细胞功能相关抗原1仅在白细胞和淋巴组织中被发现；然而，此前尚未有关于淋巴细胞功能相关抗原1在非造血细胞上表达的报道。在本研究中，对35例恶性疾病患者和36例良性疾病（包括良性肿瘤）患者的各种组织进行了淋巴细胞功能相关抗原1的免疫组化表达检测。在恶性疾病患者未被肿瘤累及的各种外分泌组织（如胃腺、支气管上皮、肺泡上皮、十二指肠腺、胆管、胰腺腺泡和唾液腺）中发现了淋巴细胞功能相关抗原1的表达。淋巴细胞功能相关抗原1的定位仅限于外分泌腺，与组织浸润白细胞不同。在所有35例接受检查的恶性疾病病例中均证实了淋巴细胞功能相关抗原1在外分泌组织上的表达。这些疾病包括多种类型的癌和造血系统肿瘤。相比之下，在36例接受检查的良性疾病病例中，无一例在外分泌腺上表达淋巴细胞功能相关抗原1。这些结果表明外分泌腺上淋巴细胞功能相关抗原1的表达与恶性疾病之间存在密切关联。在非造血细胞系中进一步证实了淋巴细胞功能相关抗原1在非造血细胞上的表达。在19个接受检查的非造血细胞系（MKN45和PANC - 1；外分泌腺细胞系）中，有两个在细胞表面和细胞质中均表达淋巴细胞功能相关抗原1。这些结果提示，通过免疫组化定义的非肿瘤性外分泌腺细胞上的淋巴细胞功能相关抗原1分子是恶性疾病存在的潜在标志物。
