Vinča Institute of Nuclear Sciences, University of Belgrade, P.O.B. 522, 11001 Belgrade, Serbia.
Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 84541 Bratislava, Slovakia.
Photodiagnosis Photodyn Ther. 2019 Jun;26:342-349. doi: 10.1016/j.pdpdt.2019.04.019. Epub 2019 Apr 22.
Despite great efforts, the design of antibacterial surfaces is still a challenge. In this work, results of structural, mechanical, cytotoxic and antibacterial activities of hydrophobic carbon quantum dots/polydimethylsiloxane surfaces are presented. Antibacterial action of this surface is based on the generation of reactive oxygen species which cause bacteria damage by oxidative stress. At the same time, this surface was not cytotoxic towards the NIH/3T3 cells. Swelling-encapsulation-shrink method is applied for encapsulation of hydrophobic carbon quantum dots in medical grade silicone-polydimethylsiloxane. XPS and photoluminescence spectroscopy analyses confirm that hydrophobic carbon quantum dots have been encapsulated successfully into polydimethylsiloxane polymer matrix. Based on stress-strain test the improvement of mechanical properties of these nanocomposites is established. It is shown by electron paramagnetic resonance spectroscopy and luminescence method that nanocomposite generates singlet oxygen initiated by 470 nm blue light irradiation. Antibacterial testing shows the nanocomposite in the form of foil kills Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae and is very effective after only a 15 min irradiation.
尽管付出了巨大努力，但抗菌表面的设计仍然是一个挑战。在这项工作中，介绍了疏水性碳量子点/聚二甲基硅氧烷表面的结构、机械、细胞毒性和抗菌活性的结果。该表面的抗菌作用基于活性氧的产生，活性氧通过氧化应激导致细菌损伤。同时，该表面对 NIH/3T3 细胞没有细胞毒性。溶胀-包封-收缩法用于将疏水性碳量子点包封在医用级硅橡胶-聚二甲基硅氧烷中。XPS 和光致发光光谱分析证实，疏水性碳量子点已成功包封到聚二甲基硅氧烷聚合物基质中。通过应力-应变测试确定了这些纳米复合材料机械性能的提高。电子顺磁共振和发光法表明，纳米复合材料在 470nm 蓝光照射下产生由单重态氧引发的单线态氧。抗菌测试表明，以箔形式存在的纳米复合材料可杀死金黄色葡萄球菌、大肠杆菌和肺炎克雷伯菌，仅照射 15 分钟后就非常有效。
