1 Shandong Institute of Pomology, Tai'an, China.
2 Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China.
Plant Dis. 2019 Jul;103(7):1458-1463. doi: 10.1094/PDIS-07-18-1214-RE. Epub 2019 Apr 25.
is an important fungal pathogen that causes apple ring rot, which can significantly reduce apple yield. Fungicide applications are the main control measure of apple ring rot worldwide. Pyraclostrobin is a quinone outside inhibitor (QoI) fungicide that has yet to be registered for control of in China. Baseline sensitivity of to pyraclostrobin (EC of mycelial growth inhibition) was assessed for 97 isolates collected in Shandong Province. The EC values ranged from 0.7010 to 7.1378 μg/ml with the mean value of 3.0870 μg/ml and displayed a unimodal frequency distribution. After cultured on fungicide-free PDA medium or on apples for multiple generations, the -resistant isolates (RS) remained resistant to pyraclostrobin, but exhibited similar virulence as the susceptible isolates (S). Cross-resistance investigation revealed that pyraclostrobin was not cross-resistant to tebuconazole, flusilazole, carbendazim, and iprodione. Field evolution showed that pyraclostrobin at 200 and 250 g a.i./ha provided greater than 80% control efficacy against apple ring rot disease when applied as a therapeutic or preventive fungicide. The efficacy was similar to fungicides that have been registered for apple.
是一种重要的真菌病原体，可引起苹果轮纹病，严重降低苹果产量。在全球范围内，使用杀菌剂是防治苹果轮纹病的主要措施。吡唑醚菌酯是一种尚未在中国登记用于防治的醌外抑制剂（QoI）杀菌剂。本研究评估了采自山东省的 97 个分离物对吡唑醚菌酯的基础敏感性（菌丝生长抑制的 EC 值）。EC 值范围为 0.7010 至 7.1378μg/ml，平均值为 3.0870μg/ml，呈单峰频率分布。在无杀菌剂的 PDA 培养基上或苹果上连续培养多代后，抗药性分离物（RS）仍对吡唑醚菌酯表现出抗性，但与敏感性分离物（S）具有相似的毒力。交叉抗性研究表明，吡唑醚菌酯与戊唑醇、氟硅唑、多菌灵和异菌脲无交叉抗性。田间进化表明，当作为治疗性或预防性杀菌剂使用时，吡唑醚菌酯在 200 和 250 g a.i./ha 下对苹果轮纹病的防治效果大于 80%，与已登记用于苹果的杀菌剂相当。
