Sun Meng, Duan Yanxin, Liu Jun Ping, Fu Jing, Huang Yonghong
College of Horticulture, Qingdao Agricultural University, Qingdao, China.
Laboratory of Quality and Safety Risk Assessment for Fruit (Qingdao), Ministry of Agriculture and Rural Affairs, Qingdao, China.
Front Microbiol. 2022 Feb 7;13:796167. doi: 10.3389/fmicb.2022.796167. eCollection 2022.
Apple ring rot caused by is prevalent in main apple-producing areas in China, bringing substantial economic losses to the growers. In the present study, we demonstrated the inhibitory effect of dimethyl trisulfide (DT), one of the main activity components identified in Chinese leek () volatile, on the apple ring rot on postharvest fruits. In experiment, 250 μL/L DT completely suppressed the mycelia growth of . In experiment, 15.63 μL/L DT showed 97% inhibition against the apple ring rot on postharvest fruit. In addition, the soluble sugar content, vitamin C content, and the soluble sugar/titratable acidity ratio of the DT-treated fruit were significantly higher than those of the control fruit. On this basis, we further explored the preliminary underlying mechanism. Microscopic observation revealed that DT seriously disrupted the normal morphology of . qRT-PCR determination showed the defense-related genes in DT-treated fruit were higher than those in the control fruit by 4.13-296.50 times, which showed that DT inhibited apple ring rot on postharvest fruit by suppressing the growth of , and inducing the defense-related genes in apple fruit. The findings of this study provided an efficient, safe, and environment-friendly alternative to control the apple ring rot on apple fruit.
由[病原体名称未给出]引起的苹果轮纹病在中国主要苹果产区普遍存在，给果农带来了巨大的经济损失。在本研究中，我们证明了二甲基三硫（DT）（中国韭菜挥发性成分中鉴定出的主要活性成分之一）对采后果实苹果轮纹病的抑制作用。在[实验名称未给出]实验中，250μL/L的DT完全抑制了[病原体名称未给出]的菌丝生长。在[实验名称未给出]实验中，15.63μL/L的DT对采后果实苹果轮纹病的抑制率达97%。此外，经DT处理的果实的可溶性糖含量、维生素C含量以及可溶性糖/可滴定酸度比均显著高于对照果实。在此基础上，我们进一步探究了初步的潜在机制。显微镜观察表明，DT严重破坏了[病原体名称未给出]的正常形态。qRT-PCR测定显示，经DT处理的果实中与防御相关的基因比对照果实高4.13 - 296.50倍，这表明DT通过抑制[病原体名称未给出]的生长并诱导苹果果实中的防御相关基因来抑制采后果实的苹果轮纹病。本研究结果为控制苹果果实的苹果轮纹病提供了一种高效、安全且环保的替代方法。
