Huang Yan, Sun Cuicui, Guan Xiangnan, Lian Sen, Li Baohua, Wang Caixia
Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.
Knight Cancer Institute, Oregon Health & Science University, Portland, OR, United States.
Front Microbiol. 2021 Jan 14;11:599062. doi: 10.3389/fmicb.2020.599062. eCollection 2020.
Apple ring rot caused by is an important disease in China, which leads to serious economic losses during storage. Plant activators are compounds that induce resistance against pathogen infection and are considered as a promising alternative strategy to traditional chemical treatment. In the present study, butylated hydroxytoluene (BHT), a potential plant activator, was evaluated for its induced resistance against in postharvest apple fruits. The physiological and molecular mechanisms involved in induced resistance were also explored. The results showed that BHT treatment could trigger strong resistance in apple fruits against , and the optimum concentration was 200 μmol L by immersion of fruits. BHT treatment significantly increased the activities of four defensive enzymes and alleviated lipid peroxidation by increasing antioxidant enzyme activities. In addition, salicylic acid (SA) content was enhanced by BHT treatment as well as the expression of three SA biosynthesis-related genes (, , and ) and two defense genes ( and ). Our results suggest that BHT-conferred resistance against might be mainly through increasing the activities of defense-related enzymes and activating SA signaling pathway, which may provide an alternative strategy to control apple ring rot in postharvest fruits.
由[病原体名称缺失]引起的苹果轮纹病在中国是一种重要病害，在储存期间会导致严重的经济损失。植物激活剂是能够诱导对病原体感染产生抗性的化合物，被认为是传统化学处理的一种有前景的替代策略。在本研究中，对叔丁基邻苯二酚（BHT）这种潜在的植物激活剂，评估了其对采后苹果果实中[病原体名称缺失]的诱导抗性。还探究了诱导抗性所涉及的生理和分子机制。结果表明，BHT处理可引发苹果果实对[病原体名称缺失]的强烈抗性，果实浸泡处理的最佳浓度为200μmol/L。BHT处理显著提高了四种防御酶的活性，并通过增加抗氧化酶活性减轻了脂质过氧化。此外，BHT处理还提高了水杨酸（SA）含量以及三个SA生物合成相关基因（[基因名称缺失]、[基因名称缺失]和[基因名称缺失]）和两个防御基因（[基因名称缺失]和[基因名称缺失]）的表达。我们的结果表明，BHT赋予的对[病原体名称缺失]的抗性可能主要是通过增加防御相关酶的活性和激活SA信号通路实现的，这可能为控制采后果实中的苹果轮纹病提供一种替代策略。
