活化的人T淋巴细胞与自身红细胞的玫瑰花结形成。受体和配体分子定义为CD2和淋巴细胞功能相关抗原3（LFA-3）。
Rosetting of activated human T lymphocytes with autologous erythrocytes. Definition of the receptor and ligand molecules as CD2 and lymphocyte function-associated antigen 3 (LFA-3).
作者信息
Plunkett M L, Sanders M E, Selvaraj P, Dustin M L, Springer T A
出版信息
J Exp Med. 1987 Mar 1;165(3):664-76. doi: 10.1084/jem.165.3.664.
Abstract
CD2, also known as LFA-2, T11, and the E rosette receptor, is a T lymphocyte surface protein functionally important in adhesion to target cells and T cell triggering. LFA-3 is a widely distributed cell surface protein that functions in adhesion on target cells. We find that LFA-3 is expressed on human E, and that CD2 is a receptor for LFA-3 that mediates T cell adhesion to human E. Pretreatment of T lymphocytes with CD2 mAb or of E with LFA-3 mAb inhibits rosetting. Purified CD2 molecules bind to human E and inhibit rosetting. 125I-CD2 binding to E is inhibited by LFA-3 mAb; reciprocally, binding of LFA-3 mAb to human E is inhibited by pretreatment with purified CD2. Higher concentrations of CD2 aggregate human E; aggregation is inhibited by mAb to LFA-3.
摘要
CD2，也被称为淋巴细胞功能相关抗原2（LFA - 2）、T11以及E玫瑰花结受体，是一种T淋巴细胞表面蛋白，在与靶细胞的黏附及T细胞激活过程中发挥重要功能。淋巴细胞功能相关抗原3（LFA - 3）是一种广泛分布的细胞表面蛋白，在与靶细胞的黏附中发挥作用。我们发现LFA - 3在人E细胞上表达，并且CD2是LFA - 3的受体，介导T细胞与人E细胞的黏附。用CD2单克隆抗体预处理T淋巴细胞或用LFA - 3单克隆抗体预处理E细胞可抑制玫瑰花结形成。纯化的CD2分子与人E细胞结合并抑制玫瑰花结形成。125I标记的CD2与E细胞的结合被LFA - 3单克隆抗体抑制；相反，LFA - 3单克隆抗体与人E细胞的结合被纯化的CD2预处理所抑制。更高浓度的CD2使人类E细胞聚集；聚集被抗LFA - 3单克隆抗体抑制。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
THE ROUTE OF RE-CIRCULATION OF LYMPHOCYTES IN THE RAT.大鼠淋巴细胞的再循环途径
Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:257-82. doi: 10.1098/rspb.1964.0001.
3
Characterization of autologous erythrocyte rosette-forming cells in Minnesota miniature swine.
Cell Immunol. 1981 Jan 1;57(1):175-82. doi: 10.1016/0008-8749(81)90130-1.
4
Inhibition of autologous rosette formation by monoclonal antibody to the sheep erythrocyte receptor.抗绵羊红细胞受体单克隆抗体对自身玫瑰花结形成的抑制作用。
J Immunol. 1982 Apr;128(4):1930-2.
5
Human T lymphocyte antigens as defined by monoclonal antibodies.由单克隆抗体所界定的人类T淋巴细胞抗原。
Immunol Rev. 1981;57:127-61. doi: 10.1111/j.1600-065x.1981.tb00445.x.
6
Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.与人类T淋巴细胞介导的细胞溶解相关的三种不同抗原：淋巴细胞功能相关抗原-1（LFA-1）、淋巴细胞功能相关抗原-2（LFA-2）和淋巴细胞功能相关抗原-3（LFA-3）。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7489-93. doi: 10.1073/pnas.79.23.7489.
7
Complement receptor (CR1) deficiency in erythrocytes from patients with systemic lupus erythematosus.系统性红斑狼疮患者红细胞中的补体受体（CR1）缺陷。
J Exp Med. 1982 May 1;155(5):1427-38. doi: 10.1084/jem.155.5.1427.
8
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Eur J Immunol. 1982 Jan;12(1):81-6. doi: 10.1002/eji.1830120115.
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Immunology. 1981 Dec;44(4):865-71.
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J Exp Med. 1981 Jan 1;153(1):207-12. doi: 10.1084/jem.153.1.207.
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