Carpenco Ecaterina, Ceauşu Raluca Amalia, Cimpean Anca Maria, Gaje Puşa Nela, Șaptefraţi Lilian, Fulga Veaceslav, David Valeriu, Raica Marius
Department of Histology, Cytology and Embryology, Nicolae Testemițanu State University of Medicine and Pharmacy, Chișinău, Republic of Moldova.
Laboratory of Morphology, Nicolae Testemițanu State University of Medicine and Pharmacy, Chișinău, Republic of Moldova.
In Vivo. 2019 May-Jun;33(3):743-748. doi: 10.21873/invivo.11534.
BACKGROUND/AIM: Mast cells (MCs) represent the most controversial non-malignant element of the tumor microenvironment. Our aim was to study how MCs density and distribution (intratumoral-MCit versus peritumoral-MCpt) relate to tumor grade and molecular subtypes.
MCs tryptase immunohistochemistry was performed on 80 cases of breast carcinomas.
For Luminal A tumors, a partial correlation was detected between MCit and progesterone receptor (PR) (p=0.005). Luminal B tumors showed a significant correlation between MCpt and age (p=0.009), estrogen receptor (ER) (p=0.017) and PR (p=0.035). MCit and MCpt were strongly interrelated in this subtype (p=0.002) and in triple-negative breast cancers (p=0.002). In HER2 subtype, MCpt tumors were significantly correlated with HER2 (p=0.044). In G2 tumors, MCpt correlated with ER (p=0.015) and PR (p=0.038) while in G3 tumors ER correlated with both MCit (p=0.009) and MCpt (p=0.000487) tumors.
MCs dynamics are strongly influenced by hormone receptors and HER2 status. MCit increased in aggressive tumor types and is a worse prognostic factor.
背景/目的：肥大细胞（MCs）是肿瘤微环境中最具争议的非恶性成分。我们的目的是研究MCs密度和分布（瘤内-MCit与瘤周-MCpt）与肿瘤分级和分子亚型之间的关系。
对80例乳腺癌进行MCs嗜酸性粒细胞阳离子蛋白免疫组化检测。
对于Luminal A型肿瘤，MCit与孕激素受体（PR）之间存在部分相关性（p = 0.005）。Luminal B型肿瘤显示MCpt与年龄（p = 0.009）、雌激素受体（ER）（p = 0.017）和PR（p = 0.035）之间存在显著相关性。在该亚型（p = 0.002）和三阴性乳腺癌（p = 0.002）中，MCit和MCpt密切相关。在HER2亚型中，MCpt肿瘤与HER2显著相关（p = 0.044）。在G2级肿瘤中，MCpt与ER（p = 0.015）和PR（p = 0.038）相关，而在G3级肿瘤中，ER与MCit（p = 0.009）和MCpt（p = 0.000487）肿瘤均相关。
MCs动态受到激素受体和HER2状态的强烈影响。MCit在侵袭性肿瘤类型中增加，是一个更差的预后因素。
