腰椎间盘退变疾病患者谷胱甘肽还原酶和丙二醛水平的评估
The Evaluation of Glutathione Reductase and Malondialdehyde Levels in Patients With Lumbar Disc Degeneration Disease.
作者信息
Bakirezer Selvi Duman, Yaltirik Cumhur Kaan, Kaya Ahmet Hilmi, Yilmaz Seda Gulec, Ozdogan Selcuk, Billur Deryanaz, Isbir Turgay
机构信息
Department of Molecular Medicine, Institute of Health Sciences, Yeditepe University, Istanbul, Turkey
Department of Neurosurgery, Faculty of Medicine, Yeditepe University, Istanbul, Turkey.
出版信息
In Vivo. 2019 May-Jun;33(3):811-814. doi: 10.21873/invivo.11543.
Abstract
BACKGROUND/AIM: Increased oxidative stress plays a crucial role in pathogenesis of various diseases. The present study aims to investigate glutathione reductase (GR) and malondialdehyde (MDA) enzymes as markers of oxidative stress mechanisms in lumbar disc degeneration disease (LDDD).
PATIENTS AND METHODS
The study group consisted of 39 patients diagnosed with LDD and 37 healthy individuals in the control group. The enzyme-linked immunosorbent assay (ELISA) method was used to determine serum GR and MDA levels in the two study groups.
RESULTS
Serum GR levels were significantly lower (p=0.008), while MDA levels were significantly higher in the patient group compared to the controls (p=0.025).
CONCLUSION
Oxidative stress mechanisms play a crucial role in disc degeneration and GR deficiency could be an eligible risk factor for LDDD.
摘要
背景/目的：氧化应激增加在多种疾病的发病机制中起关键作用。本研究旨在调查谷胱甘肽还原酶（GR）和丙二醛（MDA）酶作为腰椎间盘退变疾病（LDDD）氧化应激机制的标志物。
患者与方法
研究组由39例诊断为LDD的患者组成，对照组为37名健康个体。采用酶联免疫吸附测定（ELISA）法测定两组研究对象的血清GR和MDA水平。
结果
与对照组相比，患者组血清GR水平显著降低（p = 0.008），而MDA水平显著升高（p = 0.025）。
结论
氧化应激机制在椎间盘退变中起关键作用，GR缺乏可能是LDDD的一个合适危险因素。
相似文献
1
The Evaluation of Glutathione Reductase and Malondialdehyde Levels in Patients With Lumbar Disc Degeneration Disease.腰椎间盘退变疾病患者谷胱甘肽还原酶和丙二醛水平的评估
In Vivo. 2019 May-Jun;33(3):811-814. doi: 10.21873/invivo.11543.
2
Lipid Peroxidation, Reduced Glutathione, and Glutathione Peroxidase Levels in Intervertebral Discs of Patients with Lumbar Degenerative Disc Disease.腰椎退行性疾病患者椎间盘的脂质过氧化、还原型谷胱甘肽和谷胱甘肽过氧化物酶水平。
Med Sci Monit. 2024 May 24;30:e944335. doi: 10.12659/MSM.944335.
3
Vitamin D Receptor gene polymorphisms and plasma levels are associated with lumbar disc degeneration.维生素 D 受体基因多态性与血浆水平与腰椎间盘退变相关。
Sci Rep. 2019 May 24;9(1):7829. doi: 10.1038/s41598-019-44373-2.
4
Long non-coding RNA zinc finger antisense 1 expression associates with increased disease risk, elevated disease severity and higher inflammatory cytokines levels in patients with lumbar disc degeneration.长链非编码RNA锌指反义1的表达与腰椎间盘退变患者疾病风险增加、疾病严重程度升高及炎性细胞因子水平升高相关。
Medicine (Baltimore). 2019 Dec;98(52):e18465. doi: 10.1097/MD.0000000000018465.
5
Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration.腰椎间盘退变中端粒缩短和氧化应激增加。
Int J Mol Sci. 2022 Sep 4;23(17):10125. doi: 10.3390/ijms231710125.
6
Immuohistochemical score of matrix metalloproteinase-1 may indicate the severity of symptomatic cervical and lumbar disc degeneration.基质金属蛋白酶-1 的免疫组化评分可能表明有症状的颈椎和腰椎间盘退变的严重程度。
Spine J. 2020 Jan;20(1):124-137. doi: 10.1016/j.spinee.2019.08.004. Epub 2019 Aug 11.
7
Role of thymic stromal lymphopoietin in the pathogenesis of lumbar disc degeneration.胸腺基质淋巴细胞生成素在腰椎间盘退变发病机制中的作用
Medicine (Baltimore). 2017 Jul;96(30):e7516. doi: 10.1097/MD.0000000000007516.
8
The Evaluation of Proteoglycan Levels and the Possible Role of Gene (c.6423T>C) Variant in Patients with Lumbar Disc Degeneration Disease.腰椎间盘退变疾病患者中蛋白聚糖水平的评估及基因（c.6423T>C）变异的可能作用
In Vivo. 2019 Mar-Apr;33(2):413-417. doi: 10.21873/invivo.11488.
9
Cartilage intermediate layer protein promotes lumbar disc degeneration.软骨中间层蛋白促进腰椎间盘退变。
Biochem Biophys Res Commun. 2014 Apr 18;446(4):876-81. doi: 10.1016/j.bbrc.2014.03.025. Epub 2014 Mar 14.
10
Correlation between intervertebral disc degeneration, paraspinal muscle atrophy, and lumbar facet joints degeneration in patients with lumbar disc herniation.腰椎间盘突出症患者椎间盘退变、椎旁肌萎缩与腰椎小关节退变之间的相关性
BMC Musculoskelet Disord. 2017 Apr 20;18(1):167. doi: 10.1186/s12891-017-1522-4.
引用本文的文献
1
Oxidative stress: fundamentals and advances in quantification techniques.氧化应激：量化技术的基础与进展
Front Chem. 2024 Oct 7;12:1470458. doi: 10.3389/fchem.2024.1470458. eCollection 2024.
2
Lipid Peroxidation, Reduced Glutathione, and Glutathione Peroxidase Levels in Intervertebral Discs of Patients with Lumbar Degenerative Disc Disease.腰椎退行性疾病患者椎间盘的脂质过氧化、还原型谷胱甘肽和谷胱甘肽过氧化物酶水平。
Med Sci Monit. 2024 May 24;30:e944335. doi: 10.12659/MSM.944335.
3
Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration.腰椎间盘退变中端粒缩短和氧化应激增加。
Int J Mol Sci. 2022 Sep 4;23(17):10125. doi: 10.3390/ijms231710125.
4
Amine Functionalized Trimetallic Nitride Endohedral Fullerenes: A Class of Nanoparticle to Tackle Low Back/Leg Pain.胺功能化三金属氮化物包合物富勒烯：一类用于治疗下背部/腿部疼痛的纳米颗粒
ACS Appl Bio Mater. 2022 Jun 20;5(6):2943-2955. doi: 10.1021/acsabm.2c00269. Epub 2022 May 16.
5
CYT387, a JAK-Specific Inhibitor Impedes Osteoclast Activity and Oophorectomy-Induced Osteoporosis Modulating RANKL and ROS Signaling Pathways.CYT387，一种JAK特异性抑制剂，通过调节RANKL和ROS信号通路，抑制破骨细胞活性和卵巢切除诱导的骨质疏松症。
Front Pharmacol. 2022 Mar 8;13:829862. doi: 10.3389/fphar.2022.829862. eCollection 2022.
6
Naringin Inhibits Apoptosis Induced by Cyclic Stretch in Rat Annular Cells and Partially Attenuates Disc Degeneration by Inhibiting the ROS/NF-B Pathway.柚皮苷通过抑制 ROS/NF-B 通路抑制大鼠环状细胞周期性拉伸诱导的凋亡，部分减轻椎间盘退变。
Oxid Med Cell Longev. 2022 Feb 23;2022:6179444. doi: 10.1155/2022/6179444. eCollection 2022.
7
Plasma Concentration of the Lipid Peroxidation (LP) Biomarker 4-Ηydroxynonenal (4-HNE) in Benign and Cancer Patients.良性和癌症患者的脂质过氧化 (LP) 生物标志物 4-羟壬烯醛 (4-HNE) 的血浆浓度。
In Vivo. 2022 Mar-Apr;36(2):773-779. doi: 10.21873/invivo.12764.
8
Danshen Attenuates Intervertebral Disc Degeneration via Antioxidation in SD Rats.丹参通过抗氧化作用减轻 SD 大鼠椎间盘退变。
Oxid Med Cell Longev. 2020 Dec 22;2020:6660429. doi: 10.1155/2020/6660429. eCollection 2020.
9
LC-MS Quantification of Malondialdehyde-Dansylhydrazine Derivatives in Urine and Serum Samples.LC-MS 定量检测尿液和血清样本中的丙二醛-二盐酸肼衍生物。
J Anal Toxicol. 2020 Apr 2;44(5):470-481. doi: 10.1093/jat/bkz112.
本文引用的文献
1
Lumbar Disc Herniation.腰椎间盘突出症
Curr Rev Musculoskelet Med. 2017 Dec;10(4):507-516. doi: 10.1007/s12178-017-9441-4.
2
Understanding human thiol dioxygenase enzymes: structure to function, and biology to pathology.了解人类硫醇双加氧酶：从结构到功能，从生物学到病理学。
Int J Exp Pathol. 2017 Apr;98(2):52-66. doi: 10.1111/iep.12222. Epub 2017 Apr 24.
3
Mechanisms of low back pain: a guide for diagnosis and therapy.腰痛的机制：诊断与治疗指南
F1000Res. 2016 Jun 28;5. doi: 10.12688/f1000research.8105.2. eCollection 2016.
4
Inflammatory profiles in canine intervertebral disc degeneration.犬椎间盘退变中的炎症特征
BMC Vet Res. 2016 Jan 13;12:10. doi: 10.1186/s12917-016-0635-6.
5
Theory of MRI contrast in the annulus fibrosus of the intervertebral disc.椎间盘纤维环的磁共振成像对比理论
MAGMA. 2016 Aug;29(4):711-22. doi: 10.1007/s10334-015-0522-3. Epub 2016 Jan 11.
6
Free radicals: properties, sources, targets, and their implication in various diseases.自由基：性质、来源、靶点及其在各种疾病中的影响。
Indian J Clin Biochem. 2015 Jan;30(1):11-26. doi: 10.1007/s12291-014-0446-0. Epub 2014 Jul 15.
7
Remodelling the extracellular matrix in development and disease.重塑发育和疾病中的细胞外基质。
Nat Rev Mol Cell Biol. 2014 Dec;15(12):786-801. doi: 10.1038/nrm3904.
8
Oxidative stress participates in age-related changes in rat lumbar intervertebral discs.氧化应激参与大鼠腰椎间盘的年龄相关变化。
Arch Gerontol Geriatr. 2014 Nov-Dec;59(3):665-9. doi: 10.1016/j.archger.2014.07.002. Epub 2014 Jul 10.
9
Glutathione protects human nucleus pulposus cells from cell apoptosis and inhibition of matrix synthesis.谷胱甘肽可保护人椎间盘细胞免于细胞凋亡和基质合成抑制。
Connect Tissue Res. 2014 Apr;55(2):132-9. doi: 10.3109/03008207.2013.876421. Epub 2014 Jan 24.
10
Oswestry Disability Index is a better indicator of lumbar motion than the Visual Analogue Scale.奥斯威斯特里功能障碍指数比视觉模拟评分法更能准确反映腰椎活动情况。
Spine J. 2014 Sep 1;14(9):1860-5. doi: 10.1016/j.spinee.2013.10.027. Epub 2013 Nov 8.
Zotero 插件