Shandong Institute of Pomology, No. 64 Longtan Road, Tai'an, 271000, Shandong, China.
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A and F University, No. 3 Taicheng Road, Yangling, 712100, Shaanxi, China.
Mol Biol Rep. 2019 Aug;46(4):3755-3764. doi: 10.1007/s11033-019-04817-4. Epub 2019 Apr 26.
Ovate family proteins (OFPs), which are involved in aspects of plant development and growth, is a class of plant-specific transcription factors. Although OFPs have been reported in some species, little is known about their evolution, structure, fruit developmental expression, and interactions among OFP members in peach (Prunus persica). In this study, 15 peach OFP (PpOFP) genes were identified. Phylogenetic analysis showed that 716 OFPs from 32 species were divided into 15 subgroups; 10 subgroups (Ia, Ib, Ic, Id, Ie, If, Ig, Ih, Ii, and Ij) were composed of dicotyledonous plants only and five (IIa, IIb, IIc, IId, and IIe) were composed of monocotyledonous plants only. Structure analysis showed that the OFP genes in monocotyledonous and dicotyledonous plants had no introns. Chromosomal localization analysis showed that 15 PpOFP genes were unevenly mapped on seven chromosomes. Furthermore, eight of the 15 PpOFP genes were cloned successfully by the RT-PCR method. To some extent, eight PpOFPs were expressed in all the detected peach tissues. In addition, analysis by Y2H and BiFC technologies indicated that both PpOFP4 and PpOFP5 formed homodimers with themselves, and PpOFP5 interacted with PpOFP7 or PpOFP8 to form heterodimers. These results serve as the theoretical basis for the analysis of the biological function and regulation of peach OFP transcription factors in the growth, development and other conditions, as well as evolution studies of OFP transcription factors in higher plants.
卵形家族蛋白（OFPs）参与植物发育和生长的各个方面，是一类植物特异性转录因子。虽然在一些物种中已经报道了 OFPs，但对于它们的进化、结构、果实发育表达以及在桃（Prunus persica）中 OFP 成员之间的相互作用知之甚少。在这项研究中，鉴定了 15 个桃 OFP（PpOFP）基因。系统发育分析表明，来自 32 个物种的 716 个 OFP 分为 15 个亚组；10 个亚组（Ia、Ib、Ic、Id、Ie、If、Ig、Ih、Ii 和 Ij）仅由双子叶植物组成，而 5 个亚组（IIa、IIb、IIc、IId 和 IIe）仅由单子叶植物组成。结构分析表明，单子叶植物和双子叶植物的 OFP 基因没有内含子。染色体定位分析表明，15 个 PpOFP 基因不均匀地定位在 7 条染色体上。此外，通过 RT-PCR 方法成功克隆了 15 个 PpOFP 基因中的 8 个。在某种程度上，这 8 个 PpOFPs 在所有检测到的桃组织中都有表达。此外，通过 Y2H 和 BiFC 技术的分析表明，PpOFP4 和 PpOFP5 自身形成同源二聚体，而 PpOFP5 与 PpOFP7 或 PpOFP8 相互作用形成异源二聚体。这些结果为分析桃 OFP 转录因子在生长、发育和其他条件下的生物学功能和调控以及高等植物 OFP 转录因子的进化研究提供了理论基础。
