Kunowska Natalia, Chen Xi
Wellcome Sanger Institute, Wellcome Genome Campus, Cambridge, UK.
Methods Mol Biol. 2019;1979:269-282. doi: 10.1007/978-1-4939-9240-9_17.
Many of the key cellular processes including establishing the cell's identity are governed by chromatin proteins. Mapping their binding on the level of a single cell would give us important insights into a new dimension of cellular heterogeneity. However, ChIP-seq, the main method to study protein-DNA interaction in the chromatin context, has proven very challenging to scale down. ChIPmentation is a modification of ChIP-seq, in which the Tn5 transposase is used to introduce sequencing adapters in one step. This allows to significantly reduce the required input material. ChIPmentation is a robust and versatile approach and even though it has not yet achieved single-cell resolution, we believe that it is a very promising starting point for further downscaling.
许多关键的细胞过程，包括确定细胞的身份，都由染色质蛋白控制。在单细胞水平上绘制它们的结合图谱将为我们提供关于细胞异质性新维度的重要见解。然而，ChIP-seq作为研究染色质环境中蛋白质-DNA相互作用的主要方法，已被证明很难缩小规模。ChIPmentation是ChIP-seq的一种改进方法，其中Tn5转座酶用于一步引入测序接头。这可以显著减少所需的输入材料。ChIPmentation是一种强大且通用的方法，尽管它尚未实现单细胞分辨率，但我们认为它是进一步缩小规模的一个非常有前途的起点。
