Schmidl Christian, Rendeiro André F, Sheffield Nathan C, Bock Christoph
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.
Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria.
Nat Methods. 2015 Oct;12(10):963-965. doi: 10.1038/nmeth.3542. Epub 2015 Aug 17.
Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used to map histone marks and transcription factor binding throughout the genome. Here we present ChIPmentation, a method that combines chromatin immunoprecipitation with sequencing library preparation by Tn5 transposase ('tagmentation'). ChIPmentation introduces sequencing-compatible adaptors in a single-step reaction directly on bead-bound chromatin, which reduces time, cost and input requirements, thus providing a convenient and broadly useful alternative to existing ChIP-seq protocols.
染色质免疫沉淀测序（ChIP-seq）被广泛用于绘制全基因组范围内的组蛋白标记和转录因子结合位点。在此，我们介绍ChIPmentation，这是一种将染色质免疫沉淀与Tn5转座酶（“标签化”）进行测序文库制备相结合的方法。ChIPmentation在一步反应中直接在磁珠结合的染色质上引入与测序兼容的接头，从而减少了时间、成本和输入要求，为现有的ChIP-seq方案提供了一种方便且广泛适用的替代方法。
