高通量 ChIPmentation：从非常少量的细胞中自由扩展、单日生成 ChIPseq 数据。
High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers.
机构信息
Center for Hematology and Regenerative Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden.
Regensburg Centre for Interventional Immunology (RCI) and University Medical Center, Regensburg, Germany.
出版信息
BMC Genomics. 2019 Jan 18;20(1):59. doi: 10.1186/s12864-018-5299-0.
Abstract
BACKGROUND
Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level.
RESULTS
We present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification prior to library amplification and reduces reverse-crosslinking time from hours to minutes.
CONCLUSIONS
The resulting workflow is easily established, extremely rapid, and compatible with requirements for very low numbers of FACS sorted cells, high-throughput applications and single day data generation.
摘要
背景
染色质免疫沉淀结合测序（ChIP-seq）被广泛用于在全基因组水平上绘制组蛋白修饰和转录因子结合图谱。
结果
我们提出了高通量 ChIPmentation（HT-ChIPmentation），它消除了在文库扩增前进行 DNA 纯化的需要，并将反向交联时间从数小时缩短至数分钟。
结论
该方法具有易于建立、快速、兼容非常少量 FACS 分选细胞、高通量应用和单日数据生成等要求的特点。
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