Laboratory of Experimental Pathophysiology (LAFEx), Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Brazil.
Keenan Research Centre, Li Ka Shing Knowledge Institute, St. Michael´s Hospital, Toronto, ON, Canada; Interdepartmental Division of Critical Care Medicine, University of Toronto, Toronto, ON, Canada.
Respir Physiol Neurobiol. 2019 Aug;266:27-32. doi: 10.1016/j.resp.2019.04.013. Epub 2019 Apr 24.
Bronchial obstruction, caused by retained secretions, is often treated by the administration of mucoactive agents including distilled water, saline, hypertonic saline, and sodium bicarbonate. However, the inflammatory effect of these solutions on the lungs remains unclear. This study evaluated the instillation effects of different solutions on oxidative stress and lung inflammatory response in C57BL/6 mice. Fifty C57BL/6 mice were divided into 5 groups: control (CG); distilled water (DWG), hypertonic saline (HSG), saline (SG) and sodium bicarbonate (SBG). CG was exposed to ambient air while DWG, HSG, SG and SBG had 50 μl of respective solutions administered intranasally for 5 consecutive days. Twenty-four hours after the last intranasal instillation, all animals were euthanized for subsequent analysis. All solutions promoted increased recruitment of inflammatory cells to the lung compared to controls. Superoxide dismutase activity was lower in HSG compared to all other groups; catalase activity was reduced in SG, while it increased in SBG and DWG compared to CG. Finally, there was an increase in the inflammatory markers TNF-α, CCL2 and IFN-γ in DWG compared to CG, SG and HSG. In conclusions, the intranasal instillation of different solutions promotes redox imbalance and inflammation on lungs of adult mice.
气道阻塞，由分泌物潴留引起，通常通过给予黏液活性剂来治疗，包括蒸馏水、生理盐水、高渗盐水和碳酸氢钠。然而，这些溶液对肺部的炎症作用仍不清楚。本研究评估了不同溶液在 C57BL/6 小鼠中的灌洗效果对氧化应激和肺炎症反应的影响。将 50 只 C57BL/6 小鼠分为 5 组：对照组（CG）；蒸馏水（DWG）、高渗盐水（HSG）、生理盐水（SG）和碳酸氢钠（SBG）。CG 暴露于环境空气中，而 DWG、HSG、SG 和 SBG 则连续 5 天每天经鼻内给予 50μl 各自的溶液。最后一次鼻内灌洗后 24 小时，所有动物均被安乐死以进行后续分析。与对照组相比，所有溶液均促进了更多的炎症细胞向肺部募集。与其他组相比，HSG 中的超氧化物歧化酶活性较低；SG 中的过氧化氢酶活性降低，而 SBG 和 DWG 中的过氧化氢酶活性则升高。最后，与 CG、SG 和 HSG 相比，DWG 中的炎症标志物 TNF-α、CCL2 和 IFN-γ 增加。总之，不同溶液的鼻腔灌洗会导致成年小鼠肺部的氧化还原失衡和炎症。
