Sakarya University, Biomedical, Magnetic and Semiconductor Materials Application & Research Center (BIMAS-RC), 54187, Sakarya, Turkey.
Sakarya Applied Sciences University, Faculty of Technology, Metallurgical and Materials Engineering Department, 54187, Sakarya, Turkey.
Mater Sci Eng C Mater Biol Appl. 2019 Aug;101:292-305. doi: 10.1016/j.msec.2019.03.078. Epub 2019 Mar 23.
A novel implant coating material containing graphene oxide (GO) and collagen (COL), and hydroxyapatite (HA) was fabricated with the aid of tannic acid by electrodeposition. The surface of Ti16Nb alloy was subjected to anodic oxidation, and then HA-GO coating was applied to Ti16Nb surface by cathodic method. Then, COL was deposited on the surface of the HA-GO coating by the biomimetic method. HA, HA-GO, HA-GO-COL coatings on the surface of the Ti16Nb alloy have increased the corrosion resistance by the formation of a barrier layer on the surface. For HA-GO-COL coating, the highest corrosion resistance is obtained due to the compactness and homogeneity of the coating structure. The contact angle of the bare Ti16Nb is approximately 65°, while the contact angle of the coated samples is close to 0°. Herein, the increased surface wettability is important for cell adhesion. The surface roughness of the uncoated Ti16Nb alloy was between 1 and 3 μm, while the surface roughness of the coated surfaces was measured between 20 and 110 μm. The contact between the bone and the implant has been improved. Graphene oxide-containing coatings have improved the antibacterial properties compared to the GO-free coating using S. aureus. The hardness and elastic modulus of the coatings were measured by the nanoindentation test, and the addition of GO and collagen to the HA coating resulted in an increase in strength. The addition of GO to the HA coating reduced the viability of 3 T3 fibroblast cells, whereas the addition of collagen to HA-GO coat increased the cell adhesion and viability.
一种新型植入涂层材料，含有氧化石墨烯（GO）和胶原蛋白（COL）以及羟基磷灰石（HA），通过单宁酸辅助电沉积制备。Ti16Nb 合金表面进行阳极氧化，然后通过阴极法在 Ti16Nb 表面施加 HA-GO 涂层。然后，通过仿生方法在 HA-GO 涂层表面沉积 COL。HA、HA-GO、HA-GO-COL 涂层在 Ti16Nb 合金表面提高了耐腐蚀性，在表面形成了阻挡层。对于 HA-GO-COL 涂层，由于涂层结构的致密性和均一性，获得了最高的耐腐蚀性。Ti16Nb 的接触角约为 65°，而涂层样品的接触角接近 0°。在此，表面润湿性的增加对于细胞附着很重要。未涂层 Ti16Nb 合金的表面粗糙度在 1 到 3 μm 之间，而涂层表面的表面粗糙度在 20 到 110 μm 之间。改善了骨与植入物之间的接触。与不含 GO 的涂层相比，含 GO 的涂层提高了对金黄色葡萄球菌的抗菌性能。通过纳米压痕试验测量了涂层的硬度和弹性模量，并且将 GO 和胶原蛋白添加到 HA 涂层中导致强度增加。GO 加入到 HA 涂层中降低了 3T3 成纤维细胞的活力，而胶原蛋白加入到 HA-GO 涂层中增加了细胞的粘附和活力。
