Isakova Jainagul, Vinnikov Denis, Bukuev Nurbek, Talaibekova Elnura, Aldasheva Nazira
Institute of Molecular Biology and Medicine, Bishkek, Kazakhstan. Email:
School of Public Health, Al-Farabi Kazakh National University, Almaty, Kazakhstan.
Asian Pac J Cancer Prev. 2019 Apr 29;20(4):1057-1062. doi: 10.31557/APJCP.2019.20.4.1057.
Background: The aim of this study was to ascertain the magnitude of association of gene ТР53 Arg72Pro polymorphic marker with cervical cancer (CC) in Kyrgyz women. Methods: We identified and included 205 women of Kyrgyz ethnicity for this case-control study, of whom N=103 were women (mean age 53.5 ± 10.0 years) with histologically confirmed CC and N=102 controls (mean age 46.5 ± 8.5 years). We detected human papilloma virus (HPV) DNA types 16 and 18 using polymerase chain reaction (PCR) with hybridization/fluorescent detection. Genotypes of ТР53 gene Arg72Pro polymorphism were identified using PCR-RFLP assay. Results: Eighty-eight percent (90/103) women with CC had HPV, of whom 43.4% (39/90) had HPV type 16, 24.4% (22/90) had HPV type 18, whereas 32.2% (29/90) carried both types. The univariate analysis of allele and genotype distribution of Arg72Pro polymorphic marker of ТР53 gene showed no difference between CC and control groups (χ2=1.24, р=0.54). However, when CC cases associated with HPV were tested against controls, Arg72 allele and Arg72Arg genotype prevalence were greater compared to controls (χ²=7.25; р=0.027 for genotypes and χ²=6.83; р=0.009 for alleles). In HPV-positive women, Arg72Arg genotype of ТР53 gene was associated with a 1.85-fold increase in the likelihood of CC (OR=1.85 [95% confidence interval (CI) 1.03-3.32]), whereas Arg72 allele increased this likelihood 1.94-fold (OR=1.94 [95% CI 1.20-3.15]). Conclusions: Arg72Arg genotype and Arg72 allele of ТР53 gene in Kyrgyz women increase the risk of HPV-associated CC.
本研究旨在确定吉尔吉斯族女性中基因ТР53 Arg72Pro多态性标记与宫颈癌（CC）的关联程度。方法：我们为这项病例对照研究确定并纳入了205名吉尔吉斯族女性，其中N = 103名女性（平均年龄53.5±10.0岁）经组织学确诊为CC，N = 102名对照（平均年龄46.5±8.5岁）。我们使用聚合酶链反应（PCR）结合杂交/荧光检测来检测人乳头瘤病毒（HPV）16型和18型DNA。使用PCR-RFLP分析法鉴定ТР53基因Arg72Pro多态性的基因型。结果：88%（90/103）的CC女性感染了HPV，其中43.4%（39/90）感染了HPV 16型，24.4%（22/90）感染了HPV 18型，而32.2%（29/90）同时携带这两种类型。对ТР53基因Arg72Pro多态性标记的等位基因和基因型分布进行单因素分析，结果显示CC组和对照组之间无差异（χ2 = 1.24，p = 0.54）。然而，当将与HPV相关的CC病例与对照组进行比较时，Arg72等位基因和Arg72Arg基因型的患病率高于对照组（基因型χ² = 7.25；p = 0.027，等位基因χ² = 6.83；p = 0.009）。在HPV阳性女性中，ТР53基因的Arg72Arg基因型与CC发生可能性增加1.85倍相关（OR = 1.85 [95%置信区间（CI）1.03 - 3.32]），而Arg72等位基因使这种可能性增加1.94倍（OR = 1.94 [95% CI 1.20 - 3.15]）。结论：吉尔吉斯族女性中ТР53基因的Arg72Arg基因型和Arg72等位基因会增加HPV相关CC的风险。
