Department of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Dhaka, Bangladesh.
Department of Pharmacy, Brac University, Dhaka, Bangladesh.
PLoS One. 2021 Dec 28;16(12):e0261984. doi: 10.1371/journal.pone.0261984. eCollection 2021.
Differential expression of p53 has been reported in cervical cancer, primarily in tumor tissue biopsies. In this study, we examined the association of TP53 codon 47 and codon 72 polymorphisms and serum level expression of p53 in cervical cancer patients (n = 129) and healthy controls (n = 122). We found elevated levels of serum p53 protein levels in cervical cancer patients (p = 0.0442) compared to healthy controls. Moreover, we found higher levels of serum p53 in patients with grade-III tumor (p = 0.001) compared to healthy controls. Examination of SNPs showed TP53 Arg/Pro heterozygosity (adjusted OR = 2.126, 95% CI = 1.181-3.827, p = 0.012), Pro/Pro mutant homozygosity (adjusted OR = 3.564, 95% CI = 1.647-7.713, p = 0.001), along with the combined genotype (Arg/Pro+Pro/Pro) (adjusted OR 2.542, 95% CI = 1.517-4.260, p<0.001) significantly increases the risk of cervical cancer. Expression quantitative trait analysis revealed no significant association with protein expression. Our results represent for the first time the upregulation of serum p53 in cervical cancer in Bangladeshi women and supports the association of TP53 codon 72 polymorphisms with cervical cancer.
p53 的表达差异已在宫颈癌中报道，主要在肿瘤组织活检中。在这项研究中，我们检查了 TP53 密码子 47 和密码子 72 多态性与宫颈癌患者（n=129）和健康对照者（n=122）血清 p53 水平表达的相关性。我们发现宫颈癌患者血清 p53 蛋白水平升高（p=0.0442），与健康对照组相比。此外，我们发现 3 级肿瘤患者的血清 p53 水平更高（p=0.001），与健康对照组相比。对 SNP 的检查显示 TP53 Arg/Pro 杂合性（调整后的 OR=2.126，95%CI=1.181-3.827，p=0.012），Pro/Pro 突变纯合性（调整后的 OR=3.564，95%CI=1.647-7.713，p=0.001），以及联合基因型（Arg/Pro+Pro/Pro）（调整后的 OR=2.542，95%CI=1.517-4.260，p<0.001）显著增加了宫颈癌的风险。表达数量性状分析显示与蛋白表达无显著相关性。我们的研究结果首次代表了孟加拉国妇女宫颈癌中血清 p53 的上调，并支持 TP53 密码子 72 多态性与宫颈癌的相关性。
