Sanaei Masumeh, Kavoosi Fraidoon
Research Center for Non-Communicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran. Email:
Asian Pac J Cancer Prev. 2019 Apr 29;20(4):1119-1125. doi: 10.31557/APJCP.2019.20.4.1119.
Background: DNA demethylating agents and histone deacetylase inhibitors can affect reactivation of gene expression and apoptosis induction by DNA acetylation and demethylation. The aim of the present study was to analyze the effects of DNA demethylating agent genistein (GE) and histone deacetylase inhibitor valproic acid VPA), alone and combined, on hepatocellular carcinoma Hep G2 cell line. Methods: The cells were treated with various doses of genistein and valproic acid (alone and combined) and the MTT assay and flow cytometry were used to determine cell viability and apoptosis. Results: Genistein and valproic acid inhibited the growth of HepG 2 cells significantly. Result of flow cytometry demonstrated that genistein and valproic acid (alone and combined) induce apoptosis significantly in a time‑dependent manner. Conclusions: Genistein and valproic acid can significantly inhibit proliferation and induce apoptosis in HepG2 cell line. The apoptotic effects of GE in combination with VPA were more significant that of each compound alone.
DNA去甲基化剂和组蛋白脱乙酰酶抑制剂可通过DNA乙酰化和去甲基化影响基因表达的重新激活和凋亡诱导。本研究的目的是分析DNA去甲基化剂染料木黄酮（GE）和组蛋白脱乙酰酶抑制剂丙戊酸（VPA）单独及联合使用对肝癌Hep G2细胞系的影响。方法：用不同剂量的染料木黄酮和丙戊酸（单独及联合）处理细胞，采用MTT法和流式细胞术测定细胞活力和凋亡情况。结果：染料木黄酮和丙戊酸显著抑制HepG 2细胞的生长。流式细胞术结果表明，染料木黄酮和丙戊酸（单独及联合）均能以时间依赖性方式显著诱导凋亡。结论：染料木黄酮和丙戊酸可显著抑制HepG2细胞系的增殖并诱导凋亡。GE与VPA联合使用的凋亡效应比单独使用每种化合物更显著。
