Departamento de Nutrição, Centro de Ciências da Saúde, UFPE, Recife, Brazil.
Departamento de Fisiologia e Farmacologia, Centro de Biociências, UFPE, Recife, Brazil.
Nutr Neurosci. 2021 Feb;24(2):130-139. doi: 10.1080/1028415X.2019.1602987. Epub 2019 Apr 28.
Sepsis is a clinical syndrome with high morbidity and mortality. It is characterized by acute inflammatory response and oxidative stress, which is implicated in cerebral dysfunction. Murici ( (L.) Kunth) is a fruit rich in antioxidant compounds, which could be an alternative to prevent damage to tissues induced by sepsis Here, we evaluated the effects of sepsis on the propagation of cortical spreading depression (CSD) and oxidative stress, and tested the action of murici antioxidant extract in prevention against the effect of sepsis. Male Wistar rats (90-210 days, n = 40) were previously supplemented, orogastrically, with murici extract (150 mg/kg/day or 300 mg/kg/day), or an equivalent volume of the vehicle solution, for fifteen days. Then the animals were subjected to experimental sepsis through cecal ligation and perforation (CLP). Subsequently, CSD recordings were obtained and brain oxidative stress was evaluated. Sepsis decelerated CSD and increased the malondialdehyde (MDA) levels in the brain cortex of the animals. In contrast, septic rats that had been previously supplemented with murici antioxidant extract in doses of 150 and 300 mg/kg/day showed an increase in CSD propagation velocity, low levels of MDA and GSH/GSSG ratio and an increase of superoxide dismutase (SOD) activity, regardless of the dose tested. Our results demonstrate that sepsis affects brain excitability and that this effect can be prevented by murici antioxidant extract. The effects of sepsis and/or murici extract on CSD may be due to the oxidative state of the brain.
败血症是一种具有高发病率和死亡率的临床综合征。其特征为急性炎症反应和氧化应激，这与脑功能障碍有关。玛茹卡（（L.）Kunth）是一种富含抗氧化化合物的果实，可能是预防败血症引起的组织损伤的一种替代方法。在这里，我们评估了败血症对皮质扩散性抑制（CSD）传播和氧化应激的影响，并测试了玛茹卡抗氧化提取物在预防败血症作用中的作用。雄性 Wistar 大鼠（90-210 天，n = 40）预先通过口服补充玛茹卡提取物（150mg/kg/天或 300mg/kg/天）或等量的载体溶液，持续十五天。然后，通过盲肠结扎和穿孔（CLP）使动物发生实验性败血症。随后，获得 CSD 记录并评估大脑氧化应激。败血症会使 CSD 减速并增加动物大脑皮质中的丙二醛（MDA）水平。相比之下，先前以 150 和 300mg/kg/天的剂量补充玛茹卡抗氧化提取物的败血症大鼠表现出 CSD 传播速度增加、MDA 水平低、GSH/GSSG 比值增加和超氧化物歧化酶（SOD）活性增加，而与测试剂量无关。我们的结果表明，败血症会影响大脑兴奋性，而这种影响可以通过玛茹卡抗氧化提取物来预防。败血症和/或玛茹卡提取物对 CSD 的影响可能与大脑的氧化状态有关。
