Vocational School of Health Sciences, Karadeniz Technical University, 61080 Trabzon, Turkey.
Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkey.
Life Sci. 2020 May 15;249:117502. doi: 10.1016/j.lfs.2020.117502. Epub 2020 Mar 3.
Sepsis is defined as a life-threatening organ dysfunction caused by a dysregulated host response against infection that triggers systemic inflammatory response syndrome. l-theanine (LT), a glutamate derivative, is a non-protein amino acid derived from tea (Camellia sinensis), and a valuable nutraceutical product used as an additive in the food industry. This study we aimed to investigate whether LT would exert any therapeutic effect on liver and kidney tissues in Sprague Dawley rats with sepsis induced with cecal ligation and puncture (CLP).
Rats were divided into four groups; sham, CLP, CLP+LT1 (2x250 mg/kg) and CLP+LT2 (2 × 750 mg/kg). Liver and kidney tissues were subjected to histopathological examination. Apoptotic index percentages (AI%) were examined using the TUNEL method. The oxidized glutathione to total glutathione (GSSG/TGSH) ratio (as a marker of oxidative stress, levels of caspase-3 (a marker of apoptosis), glutathione peroxidase (GPx) and glutathione S-transferase (GST) (as antioxidant enzymes), inducible nitric oxide synthase (iNOS) and the tumor necrosis factor-α to Interleukin-10 ratio (TNF-α/IL-10) (as markers of inflammation) were investigated using commercial kits. Levels of malondialdehyde (MDA) (a marker of oxidative stress) were determined spectrophotometrically.
A high dose of LT exhibited more significant effects in reducing oxidative stress, inflammation and apoptosis than a low dose of LT in liver and kidney tissues with CLP-induced sepsis (p < 0.05).
Our results indicated that LT significantly and dose-dependently inhibited sepsis induced liver and kidney injury. This effect may be attributed to the antioxidant, anti-inflammatory, and anti-apoptotic activities of LT.
败血症是一种危及生命的器官功能障碍，由感染引起的宿主反应失调引起，引发全身炎症反应综合征。L-茶氨酸（LT）是一种谷氨酸衍生物，是一种源自茶（Camellia sinensis）的非蛋白质氨基酸，是一种有价值的营养保健品，用作食品工业中的添加剂。本研究旨在探讨 L-茶氨酸（LT）是否对盲肠结扎和穿刺（CLP）诱导败血症的 Sprague Dawley 大鼠的肝和肾组织发挥治疗作用。
将大鼠分为四组；假手术、CLP、CLP+LT1（2x250mg/kg）和 CLP+LT2（2x750mg/kg）。对肝和肾组织进行组织病理学检查。使用 TUNEL 法检测细胞凋亡指数百分比（AI%）。使用商业试剂盒检测氧化型谷胱甘肽与总谷胱甘肽（GSSG/TGSH）比值（作为氧化应激标志物）、半胱天冬氨酸蛋白酶-3（caspase-3）（作为凋亡标志物）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽 S-转移酶（GST）（作为抗氧化酶）、诱导型一氧化氮合酶（iNOS）和肿瘤坏死因子-α与白细胞介素-10 比值（TNF-α/IL-10）（作为炎症标志物）。使用分光光度法测定丙二醛（MDA）（氧化应激标志物）的水平。
与 CLP 诱导的败血症大鼠的肝和肾组织相比，高剂量 LT 在降低氧化应激、炎症和凋亡方面表现出更显著的效果，而低剂量 LT 则表现出更显著的效果（p<0.05）。
我们的结果表明，LT 显著且剂量依赖性地抑制了败血症引起的肝和肾损伤。这种作用可能归因于 LT 的抗氧化、抗炎和抗凋亡作用。
