Department of Food and Environmental Virology, National Veterinary Research Institute, Al. Partyzantów 57, 24-100, Puławy, Poland.
Department of Bioeconomy and Systems Analysis, Institute of Soil Science and Plant Cultivation, ul. Czartoryskich 8, 24-100, Puławy, Poland.
Vet Microbiol. 2019 May;232:105-113. doi: 10.1016/j.vetmic.2019.03.026. Epub 2019 Apr 5.
Rotaviruses of group A (RVAs) commonly occur in farm animals. In pigs, they cause acute gastrointestinal disease which is considered as significant factor of economic losses in pig farming. The aim of the study was an assessment of the prevalence of rotavirus (RV) infections in farmed pigs in Poland, genotype identification of the virus strains in conjunction with their age-related occurrence and regional (province) distribution pattern in pig herds. In total, 920 pig faecal samples were collected from pigs between the ages of one week and two years old from 131 farms. RVAs were detected using ELISA and molecular methods followed by a sequence-based identification of G (VP7) and P (VP4) virus genotypes. RV antigen was found in 377 (41%) of pig faecal samples. The correlation between pig age and frequency of RV infections was shown. In the Polish pig population, 145 RVA strains representing 33 GP genotypes were identified. Subsequent molecular analysis revealed an age-dependent and regional diversity in distribution of genotypes and virus strains. Besides typical pig RVA strains, novel strains such as G5P [34], G9P[34], and human G1P[8] were identified in this animal host. Findings from this study showed a change over time in the genotype occurrence of circulating pig RVAs in Poland. The high genetic variability of RV strains and acquisition of new virus genotypes have led to the emergence of novel, genetically distinct RVAs. The changes in the genotype occurrence of RVA strains in pigs indicate the need for their continuous epidemiological surveillance.
A 组轮状病毒（RVAs）通常存在于农场动物中。在猪中，它们会引起急性胃肠道疾病，这被认为是养猪业经济损失的重要因素。本研究的目的是评估波兰养殖猪中轮状病毒（RV）感染的流行情况，鉴定病毒株的基因型及其与年龄相关的发生和猪群的区域（省）分布模式。总共从 131 个农场的 1 周至 2 岁的猪中收集了 920 份猪粪便样本。使用 ELISA 和分子方法检测 RVAs，然后基于序列鉴定 G（VP7）和 P（VP4）病毒基因型。在 377 份（41%）猪粪便样本中发现了 RV 抗原。显示了猪年龄与 RV 感染频率之间的相关性。在波兰猪群中，鉴定出了代表 33 种 GP 基因型的 145 株 RVA 株。随后的分子分析显示基因型和病毒株的分布存在年龄依赖性和地区多样性。除了典型的猪 RVA 株外，还在这种动物宿主中鉴定出了新型株，如 G5P[34]、G9P[34]和人 G1P[8]。本研究的结果表明，波兰流行的循环猪 RVAs 的基因型发生了时间上的变化。RV 株的高遗传变异性和新病毒基因型的获得导致了新型、遗传上不同的 RVAs 的出现。猪 RVA 株基因型发生的变化表明需要对其进行持续的流行病学监测。
