MOE Joint International Research Laboratory of Animal Health and Food Safety, Institute of Immunology, Nanjing Agricultural University, Nanjing 210095, China; Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
China National Genebank-Shenzhen, BGI-Shenzhen, Shenzhen, 518083, China.
Vet Microbiol. 2019 May;232:13-21. doi: 10.1016/j.vetmic.2019.02.017. Epub 2019 Mar 29.
Porcine sapelovirus (PSV) is a causative agent of acute diarrhoea, respiratory distress, reproductive failure, and polioencephalomyelitis in swine. Here, we report the isolation, genomic sequence, and biological characterization of PSV isolated from pig diarrhoeal samples. In our study, two PSV strains were identified with a diameter of approximately 25 nm, and their full genomes were 7564 nucleotides in length. We named the strains PSV-JXXY-a2 and PSV-JXXY-c. Phylogenetic analysis showed that the two virus isolates were classified into the China cluster. Moreover, the PSV-JXXY-a2 strain could be inactivated quickly at 54℃ and adapted to grow on different cell lines of porcine, human, and baby hamster origin. Pathogenicity investigation showed that the isolated PSV could infect neonatal piglets efficiently and caused diarrhoea in piglets. Further epidemiological investigation revealed a high prevalence of PSV in pig herds, and the PSV-positive rates in pigs with diarrhoea were much higher than in asymptomatic samples in China. Together, our findings demonstrate that PSV-JXXY-a2 is pathogenic to neonatal piglets and advance knowledge on the prevalence of PSV infection.
猪传染性脑脊髓炎病毒（PSV）是一种引起猪急性腹泻、呼吸窘迫、繁殖失败和脑脊髓灰质炎的病原体。在这里，我们报告了从猪腹泻样本中分离、基因组序列和生物学特征的 PSV。在我们的研究中，鉴定出了两种直径约为 25nm 的 PSV 株，它们的全长基因组为 7564 个核苷酸。我们将这两个病毒株分别命名为 PSV-JXXY-a2 和 PSV-JXXY-c。系统进化分析表明，这两种病毒分离株被归类为中国群。此外，PSV-JXXY-a2 株在 54℃下能迅速失活，并能适应在不同的猪、人、和仓鼠来源的细胞系上生长。致病性研究表明，分离的 PSV 能有效地感染新生仔猪，并导致仔猪腹泻。进一步的流行病学调查显示，PSV 在猪群中的流行率很高，且在中国腹泻猪中的 PSV 阳性率明显高于无症状样本。总之，我们的研究结果表明 PSV-JXXY-a2 对新生仔猪具有致病性，并提高了对 PSV 感染流行率的认识。
