Vilar M J, Peralta B, García-Bocanegra I, Simon-Grifé M, Bensaid A, Casal J, Segalés J, Pina-Pedrero S
IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain; Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.
IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain.
Virus Res. 2016 Apr 2;215:42-9. doi: 10.1016/j.virusres.2016.01.019. Epub 2016 Feb 4.
The prevalence of Enterovirus G (EV-G) and Sapelovirus A (PSV-1) was investigated in Spanish swine herds by means of cross-sectional studies. Faecal samples from clinically healthy pigs were collected from six farms, and analysed by RT-PCR. The results indicated a high prevalence of EV-G detected in nearly all the animals older than 3 weeks of age. Otherwise, PSV-1 was only detected in 3-week-old piglets from one of the farms. Genetic analyses performed in the VP1 region of the EV-G indicated circulation of diverse strains in the same farm, related to genotypes G1, G2, G3, G4, G6, G9, G12, G13 and G14. Moreover, co-infection of several PSV-1 variants in the same animal was evident, typical of viral quasispecies. Evolutionary pressure analysis indicated that microevolution of PSV-1 seems to be driven by negative selection. This study gives further insights in the epidemiology of EV-G and PSV-1.
通过横断面研究调查了西班牙猪群中肠道病毒G（EV-G）和猪细小病毒A（PSV-1）的流行情况。从六个农场收集临床健康猪的粪便样本，并通过逆转录聚合酶链反应（RT-PCR）进行分析。结果表明，在几乎所有3周龄以上的动物中检测到EV-G的高流行率。否则，仅在其中一个农场的3周龄仔猪中检测到PSV-1。对EV-G的VP1区域进行的基因分析表明，同一农场中存在多种毒株的循环，与G1、G2、G3、G4、G6、G9、G12、G13和G14基因型有关。此外，同一动物中几种PSV-1变体的共同感染很明显，这是病毒准种的典型特征。进化压力分析表明，PSV-1的微进化似乎是由负选择驱动的。这项研究进一步深入了解了EV-G和PSV-1的流行病学。
