Division of Pathology, IVRI, Izatnagar, Bareilly, UP, India.
PDFMD, Mukteswar, Uttarakhand, India.
Transbound Emerg Dis. 2018 Feb;65(1):261-263. doi: 10.1111/tbed.12628. Epub 2017 Feb 27.
Porcine sapelovirus (PSV) A belongs to the genus Sapelovirus, family Picornaviridae. PSV infections in pigs have been reported from European countries, United States, Japan, China, Korea and Brazil. The virus has been isolated/detected from faeces of healthy pigs as well as those affected with diarrhoea, respiratory signs, encephalitis, skin lesions and fertility disorders. This study was planned to investigate whether PSV is prevalent among pigs in India and to characterize PSV encountered in the study population. The study revealed that five of 70 (7.14%) faecal samples were found positive for PSV using RT-PCR. Three viruses were successfully isolated from faecal samples using IB-RS-2 cell line. Complete genome sequencing and analysis of one Indian PSV isolate revealed highest homology (88%) with V13 strain from England. Phylogenetic analysis of the complete polyprotein nucleotide sequences of 14 strains of PSV classified the viruses into four distinct clades. This first report from India adds to our knowledge on genetic diversity of PSV detected so far among pigs in different countries. A large-scale surveillance of the virus is required to understand its genomic diversity and economic impact.
猪 sapelovirus (PSV) A 属于 sapelovirus 属，小 RNA 病毒科。已经从欧洲国家、美国、日本、中国、韩国和巴西报道了猪 sapelovirus 感染。该病毒已从健康猪以及患有腹泻、呼吸道症状、脑炎、皮肤损伤和生育障碍的猪的粪便中分离/检测到。本研究旨在调查 PSV 在印度猪中的流行情况，并对研究人群中遇到的 PSV 进行特征描述。研究结果显示，使用 RT-PCR 从 70 份粪便样本中发现了 5 份（7.14%）为 PSV 阳性。从粪便样本中成功分离出 3 株病毒，使用 IB-RS-2 细胞系。对从印度分离出的一株 PSV 进行全基因组测序和分析，发现与来自英国的 V13 株的同源性最高（88%）。对 14 株 PSV 完整多蛋白核苷酸序列的系统进化分析将病毒分为四个不同的分支。这是印度首次报道，增加了我们对不同国家猪中迄今为止检测到的 PSV 遗传多样性的了解。需要进行大规模的病毒监测，以了解其基因组多样性和经济影响。
