Department of Chemistry, Massachusetts Institute of Technology, 170 Albany Street, Cambridge, MA 02139, United States.
Department of Chemistry, Massachusetts Institute of Technology, 170 Albany Street, Cambridge, MA 02139, United States.
Curr Opin Struct Biol. 2019 Oct;58:183-190. doi: 10.1016/j.sbi.2019.03.027. Epub 2019 Apr 25.
The sensitivity of solid-state nuclear magnetic resonance (SSNMR) spectroscopy for structural biology is significantly increased by H detection under fast magic-angle spinning (MAS) and by dynamic nuclear polarization (DNP) from electron spins to nuclear spins. The former allows studies of the structure and dynamics of small quantities of proteins under physiological conditions, while the latter permits studies of large biomolecular complexes in lipid membranes and cells, protein intermediates, and protein conformational distributions. We highlight recent applications of these two emerging SSNMR technologies and point out areas for future development.
固态核磁共振（SSNMR）光谱学的灵敏度通过在快速魔角旋转（MAS）下的 H 检测以及电子自旋到核自旋的动态核极化（DNP）显著提高。前者允许在生理条件下研究少量蛋白质的结构和动力学，而后者允许在脂质膜和细胞中研究大生物分子复合物、蛋白质中间物和蛋白质构象分布。我们重点介绍了这两种新兴 SSNMR 技术的最新应用，并指出了未来的发展方向。
