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人类和小鼠脊髓干细胞龛的 RNA 分析揭示了具有 MSX1 顶盖板衍生细胞的胚胎样区域化。

RNA Profiling of the Human and Mouse Spinal Cord Stem Cell Niches Reveals an Embryonic-like Regionalization with MSX1 Roof-Plate-Derived Cells.

机构信息

INSERM U1051, INM, Hopital Saint Eloi, 80 Avenue Augustin Fliche, 34091 Montpellier, France.

Inserm U 1127, CNRS UMR 7225, Sorbonne Universités, UPMC Univ Paris 06 UMR S 1127, Institut du Cerveau et de la Moelle épinière, ICM, 75013, Paris, France.

出版信息

Stem Cell Reports. 2019 May 14;12(5):1159-1177. doi: 10.1016/j.stemcr.2019.04.001. Epub 2019 Apr 25.

DOI:10.1016/j.stemcr.2019.04.001
PMID:31031189
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6524006/
Abstract

Anamniotes, rodents, and young humans maintain neural stem cells in the ependymal zone (EZ) around the central canal of the spinal cord, representing a possible endogenous source for repair in mammalian lesions. Cell diversity and genes specific for this region are ill defined. A cellular and molecular resource is provided here for the mouse and human EZ based on RNA profiling, immunostaining, and fluorescent transgenic mice. This uncovered the conserved expression of 1,200 genes including 120 transcription factors. Unexpectedly the EZ maintains an embryonic-like dorsal-ventral pattern of expression of spinal cord developmental transcription factors (ARX, FOXA2, MSX1, and PAX6). In mice, dorsal and ventral EZ cells express Vegfr3 and are derived from the embryonic roof and floor plates. The dorsal EZ expresses a high level of Bmp6 and Gdf10 genes and harbors a subpopulation of radial quiescent cells expressing MSX1 and ID4 transcription factors.

摘要

无脊椎动物、啮齿动物和幼年人类在脊髓中央管周围的室管膜区（EZ）中维持神经干细胞，这代表了哺乳动物病变中修复的可能内源性来源。该区域的细胞多样性和特定基因尚未明确界定。本文基于 RNA 分析、免疫染色和荧光转基因小鼠，为小鼠和人类 EZ 提供了一个细胞和分子资源。这揭示了包括 120 个转录因子在内的 1200 个基因的保守表达。出乎意料的是，EZ 维持了脊髓发育转录因子（ARX、FOXA2、MSX1 和 PAX6）的胚胎样背腹表达模式。在小鼠中，背侧和腹侧 EZ 细胞表达 Vegfr3，并且来源于胚胎的顶壁和基板。背侧 EZ 表达高水平的 Bmp6 和 Gdf10 基因，并含有一个表达 MSX1 和 ID4 转录因子的静止细胞亚群。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f4/6524006/ccd05b2baf78/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f4/6524006/ac5e55667532/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f4/6524006/7d29c3412057/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f4/6524006/840febae28fb/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f4/6524006/79a8acb1c9c7/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f4/6524006/b70eb57cab7f/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f4/6524006/29be482b8378/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f4/6524006/ccd05b2baf78/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f4/6524006/ac5e55667532/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f4/6524006/7d29c3412057/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f4/6524006/840febae28fb/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f4/6524006/79a8acb1c9c7/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f4/6524006/b70eb57cab7f/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f4/6524006/29be482b8378/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f4/6524006/ccd05b2baf78/gr6.jpg

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The spinal ependymal zone as a source of endogenous repair cells across vertebrates.脊椎室管膜区作为脊椎动物内源性修复细胞的来源。
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