Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy.
Medical Science Department, University of Ferrara, Ferrara, Italy.
Front Immunol. 2019 Apr 12;10:793. doi: 10.3389/fimmu.2019.00793. eCollection 2019.
The P2X7 receptor (P2X7R) is a key pro-inflammatory plasma membrane receptor responsible for NLRP3 inflammasome activation and IL-1β release. Various inflammatory plasma membrane receptors (e.g., IL-1 type I receptor, TNF type I and II receptors, IL-2 receptor) are shed under different pathophysiological conditions. In the present study, we show that the full length P2X7R is released into circulation in patients as well as in healthy subjects. Blood levels of shed P2X7R (sP2X7R) correlate to those of the inflammatory marker C reactive protein (CRP). Blood sP2X7R ranged from 16.74 to 82.17 ng/L, mean ± SE 40.97 ± 3.82 ( = 26) in healthy subjects, from 33.1 to 484.0 ng/L, mean ± SE 114.78 ± 12.22 ( = 45) in patients with CRP <3 mg/L, and from 63.65 to 1092.3 ng/L, mean ± SE 204.2 ± 30.94 ( = 42) in patients with CRP >3 mg/L. sP2X7R in plasma was largely associated to microvesicles/microparticles. Peripheral blood monocytes from healthy subjects released sP2X7R upon stimulation with the semi-selective P2X7R agonist benzoyl ATP. These data show that the P2X7R can be released into circulation, and that its blood levels increase in various disease conditions.
P2X7 受体（P2X7R）是一种关键的促炎质膜受体，负责 NLRP3 炎性小体的激活和 IL-1β 的释放。各种炎性质膜受体（例如，IL-1 型受体、TNF 型 I 和 II 受体、IL-2 受体）在不同的病理生理条件下被释放。在本研究中，我们表明全长 P2X7R 可在患者和健康受试者中释放到循环中。释放的 P2X7R（sP2X7R）的血液水平与炎症标志物 C 反应蛋白（CRP）相关。健康受试者的血液 sP2X7R 范围为 16.74 至 82.17ng/L，平均值 ± SE 为 40.97 ± 3.82（n = 26），CRP<3mg/L 的患者为 33.1 至 484.0ng/L，平均值 ± SE 为 114.78 ± 12.22（n = 45），CRP>3mg/L 的患者为 63.65 至 1092.3ng/L，平均值 ± SE 为 204.2 ± 30.94（n = 42）。血浆中的 sP2X7R 主要与微泡/微粒相关。健康受试者的外周血单核细胞在受到半选择性 P2X7R 激动剂苯甲酰基 ATP 刺激时释放 sP2X7R。这些数据表明 P2X7R 可以释放到循环中，并且其血液水平在各种疾病情况下增加。
