miR-338-3p表达下调和MACC1表达上调提示上皮性卵巢癌患者预后不良。
Down-regulation of miR-338-3p and Up-regulation of MACC1 Indicated Poor Prognosis of Epithelial Ovarian Cancer Patients.
作者信息
Zhang Ruitao, Shi Huirong, Ren Fang, Liu Zheying, Ji Pengcheng, Zhang Weiwei, Wang Wenwen
机构信息
Department of Gynecology, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
出版信息
J Cancer. 2019 Feb 23;10(6):1385-1392. doi: 10.7150/jca.29502. eCollection 2019.
To detect the expression of microRNA-338-3p (miR-338-3p) and MET transcriptional regulator MACC1 (MACC1) gene in different ovarian tissues, to analyze their relationships, their correlations to the clinicopathologic characteristics of epithelial ovarian cancer and their significant to the progression of ovarian cancer. The expression of miR-338-3p and MACC1 gene in 20 specimens of normal ovarian tissues, 20 specimens of benign epithelial ovarian tumor and 65 specimens of epithelial ovarian cancer was detected by real-time PCR method. Their interrelationships and their correlations to the clinicopathologic characteristics of epithelial ovarian cancer were analyzed. Risk factors of recurrence and death were discussed by binary Logistic regression analysis. The relations between miR-338-3p and MACC1 expression and the survival of ovarian cancer were measured by Kaplan-Meier analysis. The expressions of miR-338-3p and MACC1 gene in epithelial ovarian cancer tissues were (0.331±0.038) and (0.774±0.025), significant differences were noted between epithelial ovarian cancer and normal ovarian tissues, benign ovarian tumors (F=77.916, =1.205E-18; F=77.945, =1.187E-18). In different ovarian tissues, miR-338-3p expression was negatively correlated to MACC1 expression (r = -0.968, <0.0001). In epithelial ovarian cancer, lower expression of miR-338-3p and higher expression of MACC1 were associated with more advanced FIGO stage, higher histological grade and developed lymph node metastasis. Down-regulation of miR-338-3p was related with the recurrence (=0. 005, OR=12.862, 95%CI: 2.12078.026) and death (=0. 007, OR=12.837, 95%CI: 2.20581.389) of ovarian cancer patients, which was showed by binary Logistic regression analysis. Compared to other patients, the overall survival rate and progression free survival rate of patients with lower miR-338-3p and higher MACC1 expression were obviously poorer (χ=16.955, =7.219E-5; χ=18.929, =2.828E-5). Down-regulation of miR-338-3p and up-regulation of MACC1 gene were closely related with the poor prognosis of epithelial ovarian cancer patients, which could served as bio-markers of the progression and recurrence of ovarian cancer.
检测微小RNA-338-3p（miR-338-3p）和MET转录调节因子MACC1（MACC1）基因在不同卵巢组织中的表达，分析它们之间的关系、与上皮性卵巢癌临床病理特征的相关性及其对卵巢癌进展的意义。采用实时荧光定量PCR法检测20例正常卵巢组织、20例良性上皮性卵巢肿瘤组织及65例上皮性卵巢癌组织中miR-338-3p和MACC1基因的表达。分析它们之间的相互关系及其与上皮性卵巢癌临床病理特征的相关性。通过二元Logistic回归分析探讨复发和死亡的危险因素。采用Kaplan-Meier分析评估miR-338-3p和MACC1表达与卵巢癌患者生存的关系。上皮性卵巢癌组织中miR-338-3p和MACC1基因的表达分别为（0.331±0.038）和（0.774±0.025），上皮性卵巢癌与正常卵巢组织、良性卵巢肿瘤之间差异有统计学意义（F=77.916，P=1.205E-18；F=77.945，P=1.187E-18）。在不同卵巢组织中，miR-338-3p表达与MACC1表达呈负相关（r = -0.968，P<0.0001）。在上皮性卵巢癌中，miR-338-3p低表达和MACC1高表达与国际妇产科联盟（FIGO）分期更高、组织学分级更高及发生淋巴结转移有关。二元Logistic回归分析显示，miR-338-3p下调与卵巢癌患者的复发（P=0. 005，OR=12.862，95%CI：2.12078.026）和死亡（P=0. 007，OR=12.837，95%CI：2.20581.389）相关。与其他患者相比，miR-338-3p低表达和MACC1高表达患者的总生存率和无进展生存率明显较差（χ=16.955，P=7.219E-5；χ=18.929，P=2.828E-5）。miR-338-3p下调和MACC1基因上调与上皮性卵巢癌患者的不良预后密切相关，可作为卵巢癌进展和复发的生物标志物。
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