SoukhakLari Roksana, Moezi Leila, Pirsalami Fatema, Abkar Morteza, Moosavi Maryam
Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Student Research Committee, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Basic Clin Neurosci. 2019 Mar-Apr;10(2):157-164. doi: 10.32598/bcn.9.10.255. Epub 2019 Mar 1.
There is evidence indicating that the rate of AD is lower in curry consuming populations. Then, there is an effort to elucidate if curcumin -as the main ingredient of turmeric-might affect the process of AD. However, in clinical trials of AD, a six-month curcumin treatment failed to show any progress, which might be attributable to its low bioavailability. In this line, a recent human study revealed that a more bioavailable solid lipid curcumin enhances cognition in aged adults. By the application of Bovine Serum Albumin (BSA), the current study aimed at converting curcumin to nano sizes and assessing its protective effects against scopolamine-induced passive avoidance memory retrieval deficit.
Nanocurcumin was prepared via dissolution method. Male NMRI mice (20-25 g body weight) were used. The effective doses of nanocurcumin were selected according to the initial pilot test. The mice were treated with nanocurcumin 15 or 20 mg/kg/p.o or distilled water for 10 days. The animals were habituated and trained in passive avoidance apparatus on the day 10. The retention test was performed 24 hours later. Scopolamine (1 mg/kg/i.p.) or saline was injected 30 minutes before memory retention trial.
The findings indicated that nanocurcumin in doses 15 or 20 mg/kg/p.o prevented the retrieval deficit induced by scopolamine while natural curcumin in its equivalent doses did not have such an effect. Furthermore, nanocurcumin by itself improved memory retention comparing with the control group.
These findings implied that the potential anti-amnesic effects of curcumin might be observed by producing and using its nanoformulation form.
有证据表明，食用咖喱的人群中阿尔茨海默病（AD）的发病率较低。因此，人们试图阐明姜黄素（作为姜黄的主要成分）是否可能影响AD的发病过程。然而，在AD的临床试验中，为期六个月的姜黄素治疗未能显示出任何进展，这可能归因于其低生物利用度。在这方面，最近一项人体研究表明，一种生物利用度更高的固体脂质姜黄素可增强老年人的认知能力。通过应用牛血清白蛋白（BSA），本研究旨在将姜黄素转化为纳米尺寸，并评估其对东莨菪碱诱导的被动回避记忆提取缺陷的保护作用。
通过溶解法制备纳米姜黄素。使用雄性NMRI小鼠（体重20 - 25克）。根据初始预试验选择纳米姜黄素的有效剂量。小鼠分别接受15或20毫克/千克/口服的纳米姜黄素或蒸馏水治疗10天。在第10天，让动物在被动回避装置中适应环境并进行训练。24小时后进行记忆保持测试。在记忆保持试验前30分钟注射东莨菪碱（1毫克/千克/腹腔注射）或生理盐水。
研究结果表明，15或20毫克/千克/口服剂量的纳米姜黄素可预防东莨菪碱诱导的记忆提取缺陷，而同等剂量的天然姜黄素则没有这种效果。此外，与对照组相比，纳米姜黄素本身可改善记忆保持。
这些发现表明，通过制备和使用姜黄素的纳米制剂形式，可能会观察到其潜在的抗遗忘作用。
