Tran Phat L, Luth Keaton, Wang James, Ray Coby, de Souza Anselm, Mehta Dilip, Moeller K W, Moeller C D, Reid Ted W
Departments of Ophthalmology and Visual Sciences, Texas Tech University Health Sciences Center, Lubbock, Texas, USA.
Viridis BioPharma Pvt Ltd, Mumbai, Maharashtra, India.
F1000Res. 2019 Mar 7;8:267. doi: 10.12688/f1000research.17707.1. eCollection 2019.
It is necessary to develop new strategies to protect against bacteria such as S , S , and , which contribute to tooth decay and plaque formation. Our current study investigated the efficacy of a colloidal silver gel in inhibiting biofilm formation by these principal oral bacteria . The aim of this study was to assess the efficacy of a colloidal silver gel formulation for inhibiting bacterial biofilm formation (Ag-gel) by the principal bacteria that cause plaque formation and tooth decay. The effect of Ag-gel on viability of , , was assessed by quantifying their colony forming units (CFU) in presence or absence of the test gel. The effect of this formulation on biofilm-forming ability of these bacteria was studied through scanning electron microscopy. Using the CFU assays, over 6 logs of inhibition (100%) were found for , , and for the Ag-gel-treated bacteria when compared with the control gel. In addition, the Ag-gel also inhibited biofilm formation by these three bacteria mixed together. These results were confirmed by scanning electron microscopy. The Ag-gel was effective in preventing biofilm formation by . This Ag-gel should be tested for the ability to block plaque formation in the mouth, through its use as a tooth paste.
有必要制定新的策略来抵御诸如变形链球菌、远缘链球菌等导致蛀牙和牙菌斑形成的细菌。我们目前的研究调查了一种胶体银凝胶抑制这些主要口腔细菌生物膜形成的功效。本研究的目的是评估一种胶体银凝胶制剂（Ag-凝胶）对导致牙菌斑形成和蛀牙的主要细菌抑制细菌生物膜形成的功效。通过在有或没有测试凝胶的情况下量化变形链球菌、远缘链球菌和血链球菌的菌落形成单位（CFU）来评估Ag-凝胶对它们生存能力的影响。通过扫描电子显微镜研究了该制剂对这些细菌生物膜形成能力的影响。使用CFU测定法，与对照凝胶相比，发现Ag-凝胶处理的变形链球菌、远缘链球菌和血链球菌的抑制率超过6个对数（100%）。此外，Ag-凝胶还抑制了这三种细菌混合在一起时生物膜的形成。这些结果通过扫描电子显微镜得到了证实。Ag-凝胶可有效防止变形链球菌形成生物膜。这种Ag-凝胶应该作为牙膏进行测试其在口腔中阻止牙菌斑形成的能力。
