Yang Xiu-Juan, Yang Zhi-Jun, Li Shuo, Ji Xiu-Jia, Ning Yan-Mei, Wang Yan
Gansu University of Chinese Medicine, Lanzhou 730000, China.
Key Laboratory of Chemistry and Quality for Chinese Medicine of Gansu Province, Lanzhou 730000, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2018 Jun 8;34(6):550-554. doi: 10.12047/j.cjap.5698.2018.123.
To study the intervention effects of Radix Hedysari, Radix Astragalus and compatibility of Angelica Sinensis on blood deficiency model mice induced by cyclophosphamide (CTX).
The mice were randomly divided into 7 groups, 10 mice each group. The blood deficiency model was established by CTX. The blank group and model group were treated with saline by gavage, while mice in positive group were administered with Lvjiaobuxue granule. Four dosage group were administered with Radix Hedysari, Radix Hedysari-Radix Angelica Sinensis(5:1), Radix Astragalus and Radix Astragalus-Radix Angelica Sinensis(5:1) water decoction. All the drugs were administered to mice for consecutive 7 d. The contents of red blood cell (RBC), lymphocyte(LYM), hematocrit (HCT), white blood cell (WBC), platelet (PLT) were detected by hematology analyzer, while thymus index(TI), spleen index(SI), reticulocyte (RC), marrow karyocyte (MK) were calculated, and the femur by pathological section were observed by microscope.
Compared with blank group, the contents of RBC, WBC, HCT, PLT, LYM were decreased in model group (<0.05). Compared with model group, the contents of RBC, WBC, HCT, PLT, LYM, RC and marrow karyocyte were increased in Hedysari-Angelica Sinensis(5:1) and Astragalus Angelica Sinensis(5:1) (<0.05), at the same time, the pathological damage of femur could be improved.
The effect of enrichment blood on blood deficiency model mice in Hedysari-Angelica Sinensis (5:1) and Astragalus-Angelica Sinensis(5:1) were superior to Hedysari and Astragalus.
研究红芪、黄芪及当归配伍对环磷酰胺（CTX）所致血虚模型小鼠的干预作用。
将小鼠随机分为7组，每组10只。采用CTX建立血虚模型。空白组和模型组灌胃给予生理盐水，阳性组给予驴胶补血颗粒，4个剂量组分别灌胃给予红芪、红芪-当归（5∶1）、黄芪、黄芪-当归（5∶1）水煎液。连续给药7 d。用血液分析仪检测红细胞（RBC）、淋巴细胞（LYM）、血细胞比容（HCT）、白细胞（WBC）、血小板（PLT）含量，计算胸腺指数（TI）、脾指数（SI）、网织红细胞（RC）、骨髓有核细胞（MK），取股骨做病理切片，显微镜下观察。
与空白组比较，模型组RBC、WBC、HCT、PLT、LYM含量降低（P<0.05）。与模型组比较，红芪-当归（5∶1）组、黄芪-当归（5∶1）组RBC、WBC、HCT、PLT、LYM、RC、骨髓有核细胞含量升高（P<0.05），同时股骨病理损伤得到改善。
红芪-当归（5∶1）、黄芪-当归（5∶1）对血虚模型小鼠的补血作用优于红芪、黄芪。
