Zhu Min, Duan Jin'ao, Tang Yuping, Su Shulan, Hua Yongqing, Guo Jianming, Qian Dawei
Jiangsu Key Laboratory for Traditional Chinese Medicine Formula Research, Nanjing University of Chinese Medicine, Nanjing 210046, China.
Zhongguo Zhong Yao Za Zhi. 2011 Sep;36(18):2543-7.
To investigate Siwu decoction and its composite drugs on the blood-deficiency model mice induced by acetylphenyhydrazine and cyclophosphamide.
Acetylphenyhydrazine and cyclophosphamide were used to copy the blood-deficiency model mice. Automatic hematology analyzer was used to test the peripheral hemogram. Weighting method was used to test the liver index and spleen index; Kits for ATPase test was used to test the activities of Na+ - K+ - ATPase/Ca2+ - Mg2+ - ATPase in erythrocyte membrane. Flow cytometry was used to test the bone marrow cells' cell cycle.
Angelicar Sinensis Radix and Paeoniae Radix Alba had the most effective activity on the peripheral hemogram. Paeoniae Radix Alba, the drug pair including Angelicar Sinensis Radix and the drug- group including Paeoniae Radix Alba had the most effective activity on the liver index. All the drugs, drug-pairs, drug-groups and the formula had effect on the spleen index. To the activity of Na+ - K+ - ATPase in erythrocyte membrane, Rehmanniae Radix Praeparata, the drug-pairs and drug-groups including Angelicar Sinensis Radix exhibited the most effective activity. All the drugs, drug-pairs, drug-groups and the formula had the protective effect on the damaged bone marrow cells.
Siwu decoction and its composite drugs all had effect on the blood-deficiency model mice, but the action intensity was different. Angelicar Sinensis Radix and Paeoniae Radix Alba exhibited the most effective activity to the protection of the blood-deficiency model mice.
研究四物汤及其配伍药物对乙酰苯肼和环磷酰胺所致血虚模型小鼠的影响。
采用乙酰苯肼和环磷酰胺复制血虚模型小鼠。用自动血液分析仪检测外周血象。用称重法检测肝脏指数和脾脏指数；用ATP酶检测试剂盒检测红细胞膜上Na+-K+-ATP酶/Ca2+-Mg2+-ATP酶活性。用流式细胞术检测骨髓细胞的细胞周期。
当归和白芍对外周血象的作用最为显著。白芍、含当归的药对及含白芍的药物组对肝脏指数的作用最为显著。所有药物、药对、药物组及方剂对脾脏指数均有影响。对红细胞膜上Na+-K+-ATP酶活性，熟地黄、含当归的药对及药物组作用最为显著。所有药物、药对、药物组及方剂对受损骨髓细胞均有保护作用。
四物汤及其配伍药物对血虚模型小鼠均有作用，但作用强度不同。当归和白芍对血虚模型小鼠的保护作用最为显著。
