Ravera Silvia, Ferrando Sara, Agas Dimitrios, De Angelis Nicola, Raffetto Mirco, Sabbieti Maria G, Signore Antonio, Benedicenti Stefano, Amaroli Andrea
Department of Experimental Medicine, University of Genoa, Genoa, Italy.
Laboratory of New Model Organism (NeMo LAB), Department of Earth, Environmental and Life Sciences, University of Genoa, Genoa, Italy.
J Biophotonics. 2019 Sep;12(9):e201900101. doi: 10.1002/jbio.201900101. Epub 2019 Jun 19.
Photobiomodulation (PBM) is a non-plant-cell manipulation through a transfer of energy by means of light sources at the non-ablative or thermal intensity. Authors showed that cytochrome-c-oxidase (complex IV) is the specific chromophore's target of PBM at the red (600-700 nm) and NIR (760-900 nm) wavelength regions. Recently, it was suggested that the infrared region of the spectrum could influence other chromospheres, despite the interaction by wavelengths higher than 900 nm with mitochondrial chromophores was not clearly demonstrated. We characterized the interaction between mitochondria respiratory chain, malate dehydrogenase, a key enzyme of Krebs cycle, and 3-hydroxyacyl-CoA dehydrogenase, an enzyme involved in the β-oxidation (two mitochondrial matrix enzymes) with the 1064 nm Nd:YAG (100mps and 10 Hz frequency mode) irradiated at the average power density of 0.50, 0.75, 1.00, 1.25 and 1.50 W/cm to generate the respective fluences of 30, 45, 60, 75 and 90 J/cm . Our results show the effect of laser light on the transmembrane mitochondrial complexes I, III, IV and V (adenosine triphosphate synthase) (window effects), but not on the extrinsic mitochondrial membrane complex II and mitochondria matrix enzymes. The effect is not due to macroscopical thermal change. An interaction of this wavelength with the Fe-S proteins and Cu-centers of respiratory complexes and with the water molecules could be supposed.
光生物调节（PBM）是一种通过非消融或热强度的光源进行能量转移的非植物细胞操作。作者表明，细胞色素c氧化酶（复合体IV）是PBM在红色（600 - 700nm）和近红外（760 - 900nm）波长区域的特定发色团靶点。最近，有人提出光谱的红外区域可能会影响其他色球层，尽管波长高于900nm与线粒体发色团的相互作用尚未得到明确证实。我们对线粒体呼吸链、三羧酸循环的关键酶苹果酸脱氢酶以及参与β氧化的酶3 - 羟基酰基辅酶A脱氢酶（两种线粒体基质酶）之间的相互作用进行了表征，使用平均功率密度为0.50、0.75、1.00、1.25和1.50W/cm的1064nm Nd:YAG（100mps和10Hz频率模式）进行照射，以产生30、45、60、75和90J/cm的相应能量密度。我们的结果表明激光对跨膜线粒体复合体I、III、IV和V（三磷酸腺苷合酶）有影响（窗口效应），但对线粒体外膜复合体II和线粒体基质酶没有影响。这种效应不是由于宏观热变化引起的。可以推测这种波长与呼吸复合体的铁硫蛋白和铜中心以及水分子之间存在相互作用。
