Chott A, Klöppel G, Buxbaum P, Heitz P U
Virchows Arch A Pathol Anat Histopathol. 1987;410(5):397-402. doi: 10.1007/BF00712759.
Immunoreactivity to neuron specific enolase (NSE) was demonstrated in a solid-cystic (papillary cystic) tumour of the human pancreas, employing immunohistochemical methods. Positive staining for NSE was found with two different antisera. In addition, sodium-dodecyl-sulphate-polyacrylamide-gel-electro-phoresis (SDS-PAGE) of tumour homogenate revealed a distinct band reacting with a NSE antiserum. However, we failed to detect any hormonal products or neuroendocrine granules in the tumour. Therefore the authors advise caution in using the enzyme as a differential diagnostic tool, especially in surgical pathology of epithelial pancreatic neoplasms occurring in young females. In individual cases electron microscopy will be necessary since solid-cystic tumours of the pancreas consistently show large intracytoplasmic zymogen-like granules.
采用免疫组化方法，在一例人胰腺实性囊性（乳头状囊性）肿瘤中证实了对神经元特异性烯醇化酶（NSE）的免疫反应性。用两种不同的抗血清发现了NSE的阳性染色。此外，肿瘤匀浆的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）显示有一条与NSE抗血清反应的明显条带。然而，我们未能在肿瘤中检测到任何激素产物或神经内分泌颗粒。因此，作者建议在将该酶用作鉴别诊断工具时要谨慎，尤其是在年轻女性发生的胰腺上皮性肿瘤的外科病理学中。在个别情况下，电子显微镜检查将是必要的，因为胰腺实性囊性肿瘤始终显示出大量胞浆内的类酶原颗粒。
