Zhang Yu, Yao Yupeng, Zhang Yan
MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences, Sun Yat-sen University.
MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences, Sun Yat-sen University;
J Vis Exp. 2019 Apr 12(146). doi: 10.3791/59271.
Osteosarcoma (OS) is the most common and a highly aggressive primary bone tumor. It is characterized with anatomic and histologic variations along with diagnostic or prognostic difficulties. OS comprises genotypically and phenotypically heterogeneous cancer cells. Bone microenvironment elements are proved to account for tumor heterogeneity and disease progression. Bone extracellular matrix (BEM) retains the microstructural matrices and biochemical components of native extracellular matrix. This tissue-specific niche provides a favorable and long-term scaffold for OS cell seeding and proliferation. This article provides a protocol for the preparation of BEM model and its further experimental application. OS cells can grow and differentiate into multiple phenotypes consistent with the histopathological complexity of OS clinical specimens. The model also allows visualization of diverse morphologies and their association with genetic alterations and underlying regulatory mechanisms. As homologous to human OS, this BEM-OS model can be developed and applied to the pathology and clinical research of OS.
骨肉瘤（OS）是最常见且具有高度侵袭性的原发性骨肿瘤。它具有解剖学和组织学变异，同时存在诊断或预后困难。骨肉瘤由基因型和表型异质性的癌细胞组成。骨微环境因素被证明是肿瘤异质性和疾病进展的原因。骨细胞外基质（BEM）保留了天然细胞外基质的微观结构基质和生化成分。这种组织特异性微环境为骨肉瘤细胞的接种和增殖提供了一个有利且长期的支架。本文提供了一种制备BEM模型及其进一步实验应用的方案。骨肉瘤细胞可以生长并分化为多种与骨肉瘤临床标本的组织病理学复杂性一致的表型。该模型还可以使不同形态可视化，并显示它们与基因改变和潜在调控机制的关联。作为与人类骨肉瘤同源的模型，这种BEM-OS模型可用于骨肉瘤的病理学和临床研究。
