Guangdong-Hongkong-Macau Institute of CNS Regeneration (GHMICR), MOE Joint International Research Laboratory of CNS Regeneration , Jinan University , Guangzhou , Guangdong , 510632 , China.
College of Life Science and Technology , Jinan University , Guangzhou , Guangdong , 510632 , China.
ACS Appl Mater Interfaces. 2019 May 22;11(20):18254-18267. doi: 10.1021/acsami.9b04862. Epub 2019 May 9.
Poly(3,4-ethylene dioxythiophene) (PEDOT) is a promising conductive material widely used for interfacing with tissues in biomedical fields because of its unique properties. However, obtaining high charge injection capability and high stability remains challenging. In this study, pristine carbon nanotubes (CNTs) modified by dopamine (DA) self-polymerization on the surface polydopamine (PDA@CNTs) were utilized as dopants of PEDOT to prepare hybrid films through electrochemical deposition on the indium tin oxide (ITO) electrode. The PDA@CNTs-PEDOT film of the nanotube network topography exhibited excellent stability and strong adhesion to the ITO substrate compared with PEDOT and PEDOT/ p-toulene sulfonate. The PDA@CNTs-PEDOT-coated ITO electrodes demonstrated lower impedance and enhanced charge storage capacity than the bare ITO. When applying exogenous electrical stimulation (ES), robust long neurites sprouted from the dorsal root ganglion (DRG) neurons cultured on the PDA@CNTs-PEDOT film. Moreover, ES promoted Schwann cell migration out from the DRG spheres and enhanced myelination. The PDA@CNTs-PEDOT film served as an excellent electrochemical sensor for the detection of DA in the presence of biomolecule interferences. Results would shed light into the advancement of conducting nanohybrids for applications in the multifunctional bioelectrode in neuroscience.
聚（3,4-亚乙基二氧噻吩）（PEDOT）是一种很有前途的导电材料，由于其独特的性质，广泛用于生物医学领域与组织的界面。然而，获得高电荷注入能力和高稳定性仍然具有挑战性。在这项研究中，利用多巴胺（DA）自聚合在表面聚多巴胺（PDA）上修饰的原始碳纳米管（CNTs）（PDA@CNTs）作为 PEDOT 的掺杂剂，通过电化学沉积在氧化铟锡（ITO）电极上制备混合薄膜。与 PEDOT 和 PEDOT/对甲苯磺酸盐相比，具有纳米管网络形貌的 PDA@CNTs-PEDOT 薄膜表现出优异的稳定性和对 ITO 基底的强粘附性。与裸 ITO 相比，PDA@CNTs-PEDOT 涂层 ITO 电极具有更低的阻抗和增强的电荷存储能力。当施加外源性电刺激（ES）时，在 PDA@CNTs-PEDOT 薄膜上培养的背根神经节（DRG）神经元上长出了强壮的长神经突。此外，ES 促进施万细胞从 DRG 球体中迁移并增强髓鞘形成。PDA@CNTs-PEDOT 薄膜可用作电化学传感器，用于在存在生物分子干扰的情况下检测 DA。这些结果为多功能生物电极中导电纳米复合材料的应用提供了新的思路。
