Department of Condensed Matter, Applied Physics and Nanoscience, Brazilian Center for Physics Research, Urca, Rio de Janeiro, Brazil.
Department of Cellular and Molecular Biology, Institute of Biology, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
J Biomed Mater Res A. 2019 Sep;107(9):1965-1976. doi: 10.1002/jbm.a.36709. Epub 2019 May 13.
Nanostructured carbonated hydroxyapatite (nCHA) is a promising biomaterial for bone tissue engineering due to its chemical properties, similar to those of the bone mineral phase and its enhanced in vivo bioresorption. However, the biological effects of nCHA nanoparticles on cells and tissues are not sufficiently known. This study assessed the impact of exposing pre-osteoblasts to suspensions with high doses of nCHA nanoparticles with high or low crystallinity. MC3T3-E1 pre-osteoblasts were cultured for 1 or 7 days in a culture medium previously exposed to CHA nanoparticles for 1 day. Control groups were produced by centrifugation for removal of bigger nCHA aggregates before exposure. Interaction of nanoparticles with the culture medium drastically changed medium composition, promoting Ca, P, and protein adsorption. Transmission Electron microscopy revealed that exposed cells were able to internalize both materials, which seemed concentrated inside endosomes. No cytotoxicity was observed for both materials, regardless of centrifugation, and the exposure did not induce alterations in the release of pro-and anti-inflammatory cytokines. Morphological analysis revealed strong interactions of nCHA aggregates with cell surfaces, however without marked alterations in morphological features and cytoskeleton ultrastructure. The overall in vitro biocompatibility of nCHA materials, regardless of physicochemical characteristics such as crystallinity, encourages further studies on their clinical applications.
纳米结构碳酸羟基磷灰石（nCHA）是一种很有前途的骨组织工程生物材料，因为它具有类似于骨矿物质相的化学性质和增强的体内生物吸收性。然而，nCHA 纳米颗粒对细胞和组织的生物学效应还不是很清楚。本研究评估了将高剂量高或低结晶度 nCHA 纳米颗粒悬浮液暴露于前成骨细胞的影响。MC3T3-E1 前成骨细胞在培养 1 天或 7 天的培养基中预先暴露于 CHA 纳米颗粒 1 天。对照组通过离心在暴露前去除较大的 nCHA 聚集体来制备。纳米颗粒与培养基的相互作用极大地改变了培养基的组成，促进了 Ca、P 和蛋白质的吸附。透射电子显微镜显示，暴露的细胞能够内化这两种材料，这些材料似乎集中在内体中。两种材料均未观察到细胞毒性，无论离心与否，暴露都不会诱导促炎和抗炎细胞因子释放的改变。形态分析显示 nCHA 聚集体与细胞表面强烈相互作用，但细胞形态特征和细胞骨架超微结构没有明显改变。nCHA 材料的整体体外生物相容性，无论其理化特性如结晶度如何，都鼓励对其临床应用进行进一步研究。
