Priyadharshini S Swathi, Ragavendran Chinnasamy, Sherwood Anand, Ramya J Ramana, Krithikadatta Jogikalmat
Department of Cariology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, TN, India.
Department of Conservative Dentistry and Endodontics, C.S.I. College of Dental Sciences and Research, Madurai, TN, India.
Restor Dent Endod. 2024 Oct 29;49(4):e40. doi: 10.5395/rde.2024.49.e40. eCollection 2024 Nov.
This study aimed to evaluate carbonated hydroxyapatite (CHA)'s ability for mineral induction and its cytotoxicity with human dental pulp cells.
Precursors for the study include di-ammonium hydrogen phosphate and calcium nitrate tetrahydrate, with sodium hydrogen carbonate added to achieve different levels of carbonate substitution. The synthesized CHA samples are characterized using X-ray diffraction, Fourier transform infrared spectroscopy, and Raman spectroscopy. Scanning electron microscopy (SEM) was used to observe morphology. For 14 days at 37°C, samples were submerged in simulated body fluid to assess their mineral induction capabilities. SEM was used to confirm apatite formation on sample surfaces. The cytotoxicity assay was used to assess the vitality of the cells following their exposure to various concentrations of CHA.
The Joint Committee on Powder Diffraction Standards data for HA aligned well with the results from X-ray diffraction analysis of CHA across 3 different concentrations, indicating strong agreement. Fourier transform infrared spectra indicated the presence of phosphate, hydroxyl, and carbonate groups within the samples. SEM and Energy-dispersive X-ray analysis show agglomerated and flaky nanoparticles. All the samples are bioactive, but the formation of apatite differs from one another. cytotoxicity assay showed that over 70% of cells maintain viability.
The results of this study may provide insight into the potential use of carbonated HA as a dental pulp-capping material for vital pulp therapy.
本研究旨在评估碳酸羟基磷灰石（CHA）对人牙髓细胞的矿化诱导能力及其细胞毒性。
该研究的前驱体包括磷酸氢二铵和四水合硝酸钙，并添加碳酸氢钠以实现不同程度的碳酸盐取代。使用X射线衍射、傅里叶变换红外光谱和拉曼光谱对合成的CHA样品进行表征。扫描电子显微镜（SEM）用于观察形态。在37°C下将样品浸泡在模拟体液中14天，以评估其矿化诱导能力。使用SEM确认样品表面磷灰石的形成。细胞毒性试验用于评估细胞在暴露于不同浓度的CHA后的活力。
羟基磷灰石的粉末衍射标准联合委员会数据与3种不同浓度的CHA的X射线衍射分析结果吻合良好，表明一致性很强。傅里叶变换红外光谱表明样品中存在磷酸根、羟基和碳酸根基团。SEM和能量色散X射线分析显示有团聚的片状纳米颗粒。所有样品都具有生物活性，但磷灰石的形成各不相同。细胞毒性试验表明，超过70%的细胞保持活力。
本研究结果可为碳酸化羟基磷灰石作为活髓治疗的牙髓盖髓材料的潜在应用提供见解。
