Dornelas Jessica, Dornelas Gisele, Tude Elena Mavropoulos Oliveira, Mourão Carlos Fernando, Rossi Alexandre da Malta, Alves Gutemberg Gomes
NanoOnco3D, Rio de Janeiro 24033-000, Brazil.
Cell and Molecular Biology Department, Institute of Biology, Fluminense Federal University, Niterói 24220-900, Brazil.
Materials (Basel). 2024 Aug 17;17(16):4092. doi: 10.3390/ma17164092.
The increasing demand for effective bone regeneration materials drives the exploration of biomaterials with enhanced bioactivity and biocompatibility, such as zinc-substituted compounds. This study investigates the in vitro cellular interactions with nanostructured spheres composed of alginate/carbonated hydroxyapatite (CHA), compared to zinc-substituted CHA (ZnCHA). This work aimed to compare the physicochemical properties and biological effects of ZnCHA and CHA on osteoblasts. ZnCHA was synthesized using a wet chemical method, followed by characterization through X-ray diffraction, Fourier transform infrared spectroscopy, total organic carbon analysis, Wavelength-dispersive X-ray spectroscopy, and BET surface area analysis to assess ion release and structural changes. Biological evaluation was conducted using cell viability, proliferation, and biomineralization assays on osteoblasts. Results showed successful incorporation of zinc and carbonate, leading to reduced crystallinity and increased surface area. Cell viability and proliferation assays indicated ZnCHA's cytocompatibility and enhanced osteoblastic activity, with increased mineralization nodules compared to CHA samples. The study concludes that ZnCHA composites are promising candidates for bone tissue engineering, demonstrating improved cytocompatibility and potential for further preclinical evaluations.
对有效骨再生材料日益增长的需求推动了对具有增强生物活性和生物相容性的生物材料的探索，例如锌取代化合物。本研究调查了与锌取代的碳酸羟基磷灰石（ZnCHA）相比，由藻酸盐/碳酸羟基磷灰石（CHA）组成的纳米结构球体的体外细胞相互作用。这项工作旨在比较ZnCHA和CHA对成骨细胞的物理化学性质和生物学效应。采用湿化学方法合成ZnCHA，然后通过X射线衍射、傅里叶变换红外光谱、总有机碳分析、波长色散X射线光谱和BET表面积分析进行表征，以评估离子释放和结构变化。使用成骨细胞的细胞活力、增殖和生物矿化测定进行生物学评估。结果表明成功掺入了锌和碳酸盐，导致结晶度降低和表面积增加。细胞活力和增殖测定表明ZnCHA具有细胞相容性并增强了成骨活性，与CHA样品相比，矿化结节增加。该研究得出结论，ZnCHA复合材料是骨组织工程的有前途的候选材料，显示出改善的细胞相容性和进一步进行临床前评估的潜力。
