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本文引用的文献

1
Isolation and characterization of the mouse metallothionein-I gene.小鼠金属硫蛋白-I基因的分离与鉴定
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6511-5. doi: 10.1073/pnas.77.11.6511.
2
Human metallothionein genes: molecular cloning and sequence analysis of the mRNA.人类金属硫蛋白基因:mRNA的分子克隆与序列分析
Nucleic Acids Res. 1982 May 25;10(10):3165-73. doi: 10.1093/nar/10.10.3165.
3
Cloning and sequence analysis of two monkey metallothionein cDNAs.两种猴金属硫蛋白cDNA的克隆与序列分析
Gene. 1983 Sep;24(1):137-46. doi: 10.1016/0378-1119(83)90139-7.
4
Affecting gene expression by altering the length and sequence of the 5' leader.通过改变5'前导序列的长度和序列来影响基因表达。
Proc Natl Acad Sci U S A. 1984 Dec;81(24):7698-702. doi: 10.1073/pnas.81.24.7698.
5
Role of mRNA translational efficiency in bovine growth hormone expression in Escherichia coli.信使核糖核酸翻译效率在大肠杆菌中牛生长激素表达中的作用
Proc Natl Acad Sci U S A. 1984 Sep;81(17):5403-7. doi: 10.1073/pnas.81.17.5403.
6
RNA synthesis startpoints in bacteriophage lambda: are the promoter and operator transcribed?噬菌体λ中的RNA合成起始点:启动子和操纵基因会被转录吗?
Nat New Biol. 1972 Jun 21;237(77):227-32. doi: 10.1038/newbio237227a0.
7
Metallothionein.金属硫蛋白
Annu Rev Biochem. 1986;55:913-51. doi: 10.1146/annurev.bi.55.070186.004405.
8
Maximizing gene expression from plasmid vectors containing the lambda PL promoter: strategies for overproducing transcription termination factor rho.最大化含有λPL启动子的质粒载体的基因表达:过量生产转录终止因子rho的策略。
Proc Natl Acad Sci U S A. 1985 Jan;82(1):88-92. doi: 10.1073/pnas.82.1.88.
9
Thionein gene expression in Cd++-variants of the CHO cell: correlation of thionein synthesis rates with translatable mRNA levels during induction, deinduction, and superinduction.中国仓鼠卵巢细胞镉离子变异体中硫蛋白基因的表达:诱导、去诱导和超诱导过程中硫蛋白合成速率与可翻译信使核糖核酸水平的相关性。
Nucleic Acids Res. 1979 Sep 11;7(1):271-88. doi: 10.1093/nar/7.1.271.
10
A new method for sequencing DNA.一种新的DNA测序方法。
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4. doi: 10.1073/pnas.74.2.560.

克隆的猴金属硫蛋白在大肠杆菌中的表达。

Expression of cloned monkey metallothionein in Escherichia coli.

作者信息

Murooka Y, Nagaoka T

出版信息

Appl Environ Microbiol. 1987 Jan;53(1):204-7. doi: 10.1128/aem.53.1.204-207.1987.

DOI:10.1128/aem.53.1.204-207.1987
PMID:3103531
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC203630/
Abstract

Expression vectors were constructed in which a cDNA specifying the monkey kidney metallothionein-II (MT-II) was linked directly to the lambda PR promoter. Enhanced expression of MT-II in Escherichia coli was observed when two initiation signals were tandemly linked to the MT-II gene and the lambda cI+ host cells were induced by nalidixic acid.

摘要

构建了表达载体,其中编码猴肾金属硫蛋白-II(MT-II)的cDNA直接与λPR启动子相连。当两个起始信号串联连接到MT-II基因上,并且用萘啶酸诱导λcI+宿主细胞时,观察到大肠杆菌中MT-II的表达增强。