克隆的猴金属硫蛋白在大肠杆菌中的表达。
Expression of cloned monkey metallothionein in Escherichia coli.
作者信息
Murooka Y, Nagaoka T
出版信息
Appl Environ Microbiol. 1987 Jan;53(1):204-7. doi: 10.1128/aem.53.1.204-207.1987.
Abstract
Expression vectors were constructed in which a cDNA specifying the monkey kidney metallothionein-II (MT-II) was linked directly to the lambda PR promoter. Enhanced expression of MT-II in Escherichia coli was observed when two initiation signals were tandemly linked to the MT-II gene and the lambda cI+ host cells were induced by nalidixic acid.
摘要
构建了表达载体，其中编码猴肾金属硫蛋白-II（MT-II）的cDNA直接与λPR启动子相连。当两个起始信号串联连接到MT-II基因上，并且用萘啶酸诱导λcI+宿主细胞时，观察到大肠杆菌中MT-II的表达增强。
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本文引用的文献
1
Isolation and characterization of the mouse metallothionein-I gene.小鼠金属硫蛋白-I基因的分离与鉴定
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6511-5. doi: 10.1073/pnas.77.11.6511.
2
Human metallothionein genes: molecular cloning and sequence analysis of the mRNA.人类金属硫蛋白基因：mRNA的分子克隆与序列分析
Nucleic Acids Res. 1982 May 25;10(10):3165-73. doi: 10.1093/nar/10.10.3165.
3
Cloning and sequence analysis of two monkey metallothionein cDNAs.两种猴金属硫蛋白cDNA的克隆与序列分析
Gene. 1983 Sep;24(1):137-46. doi: 10.1016/0378-1119(83)90139-7.
4
Affecting gene expression by altering the length and sequence of the 5' leader.通过改变5'前导序列的长度和序列来影响基因表达。
Proc Natl Acad Sci U S A. 1984 Dec;81(24):7698-702. doi: 10.1073/pnas.81.24.7698.
5
Role of mRNA translational efficiency in bovine growth hormone expression in Escherichia coli.信使核糖核酸翻译效率在大肠杆菌中牛生长激素表达中的作用
Proc Natl Acad Sci U S A. 1984 Sep;81(17):5403-7. doi: 10.1073/pnas.81.17.5403.
6
RNA synthesis startpoints in bacteriophage lambda: are the promoter and operator transcribed?噬菌体λ中的RNA合成起始点：启动子和操纵基因会被转录吗？
Nat New Biol. 1972 Jun 21;237(77):227-32. doi: 10.1038/newbio237227a0.
7
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Annu Rev Biochem. 1986;55:913-51. doi: 10.1146/annurev.bi.55.070186.004405.
8
Maximizing gene expression from plasmid vectors containing the lambda PL promoter: strategies for overproducing transcription termination factor rho.最大化含有λPL启动子的质粒载体的基因表达：过量生产转录终止因子rho的策略。
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9
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Nucleic Acids Res. 1979 Sep 11;7(1):271-88. doi: 10.1093/nar/7.1.271.
10
A new method for sequencing DNA.一种新的DNA测序方法。
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4. doi: 10.1073/pnas.74.2.560.
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